Millennium Institute on Immunology and Immunotherapy, Santiago, Chile.
Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile.
J Infect Dis. 2023 Oct 3;228(7):857-867. doi: 10.1093/infdis/jiad320.
We sought to identify potential antigens for discerning between humoral responses elicited after vaccination with CoronaVac (a severe acute respiratory syndrome coronavirus 2 [SARS-CoV-2] inactivated vaccine), natural infection, or breakthrough infection.
Serum samples obtained from volunteers immunized with CoronaVac (2 and 3 doses), breakthrough case patients, and from convalescent individuals were analyzed to determine the immunoglobulin (Ig) G responses against 3 structural and 8 nonstructural SARS-CoV-2 antigens.
Immunization with CoronaVac induced higher levels of antibodies against the viral membrane (M) protein compared with convalescent subjects both after primary vaccination and after a booster dose. Individuals receiving a booster dose displayed equivalent levels of IgG antibodies against the nucleocapsid (N) protein, similar to convalescent subjects. Breakthrough case patients produced the highest antibody levels against the N and M proteins. Antibodies against nonstructural viral proteins were present in >50% of the convalescent subjects.
Vaccinated individuals elicited a different humoral response compared to convalescent subjects. The analysis of particular SARS-CoV-2 antigens could be used as biomarkers for determining infection in subjects previously vaccinated with CoronaVac.
我们旨在确定潜在的抗原，以区分接种科兴疫苗（一种严重急性呼吸综合征冠状病毒 2 [SARS-CoV-2] 灭活疫苗）、自然感染或突破性感染后产生的体液反应。
分析了接种科兴疫苗（2 剂和 3 剂）、突破性病例患者和恢复期个体的血清样本，以确定针对 3 种结构和 8 种非结构 SARS-CoV-2 抗原的免疫球蛋白（IgG）反应。
与恢复期个体相比，接种科兴疫苗后初次接种和加强接种均可诱导针对病毒膜（M）蛋白的抗体水平升高。接受加强剂量的个体对核衣壳（N）蛋白的 IgG 抗体水平与恢复期个体相当。突破性病例患者对 N 和 M 蛋白产生的抗体水平最高。针对非结构病毒蛋白的抗体存在于 >50%的恢复期个体中。
与恢复期个体相比，接种个体产生了不同的体液反应。分析特定的 SARS-CoV-2 抗原可以作为以前接种过科兴疫苗的个体确定感染的生物标志物。
