Department of Chemistry, University of Illinois at Urbana-Champaign, 600 S Mathews Avenue, Urbana, Illinois, US.
The Imaging Technology Group, Beckman Institute for Advanced Science & Technology, University of Illinois at Urbana-Champaign, 405 North Mathews Avenue, Urbana, IL, US.
Nat Commun. 2024 Jul 16;15(1):5947. doi: 10.1038/s41467-024-49585-3.
Conversion of heterotrophic organisms into partially or completely autotrophic organisms is primarily accomplished by extensive metabolic engineering and laboratory evolution efforts that channel CO into central carbon metabolism. Here, we develop a directed endosymbiosis approach to introduce carbon assimilation in budding yeasts. Particularly, we engineer carbon assimilating and sugar-secreting photosynthetic cyanobacterial endosymbionts within the yeast cells, which results in the generation of yeast/cyanobacteria chimeras that propagate under photosynthetic conditions in the presence of CO and in the absence of feedstock carbon sources like glucose or glycerol. We demonstrate that the yeast/cyanobacteria chimera can be engineered to biosynthesize natural products under the photosynthetic conditions. Additionally, we expand our directed endosymbiosis approach to standard laboratory strains of yeasts, which transforms them into photosynthetic yeast/cyanobacteria chimeras. We anticipate that our studies will have significant implications for sustainable biotechnology, synthetic biology, and experimentally studying the evolutionary adaptation of an additional organelle in yeast.
将异养生物转化为部分或完全自养生物主要通过广泛的代谢工程和实验室进化努力来实现，这些努力将 CO 导入中心碳代谢。在这里，我们开发了一种定向内共生方法来引入芽殖酵母中的碳同化。具体来说，我们在酵母细胞内工程化了具有碳同化和糖分泌功能的光合蓝藻内共生体，这导致产生了在 CO 存在下和在葡萄糖或甘油等原料碳源不存在的情况下在光合作用条件下繁殖的酵母/蓝藻嵌合体。我们证明可以对酵母/蓝藻嵌合体进行工程改造，使其在光合作用条件下生物合成天然产物。此外，我们将我们的定向内共生方法扩展到酵母的标准实验室菌株，将它们转化为光合酵母/蓝藻嵌合体。我们预计我们的研究将对可持续生物技术、合成生物学以及在酵母中实验研究额外细胞器的进化适应具有重要意义。
