Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization, Nasushiobara, Tochigi 329-2793, Japan.
Department of Informatics, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan.
Int J Syst Evol Microbiol. 2024 Jul;74(7). doi: 10.1099/ijsem.0.006456.
A Gram-stain-negative, endospore-forming, rod-shaped, indole-producing bacterial strain, designated YZC6, was isolated from fermented cabbage. Strain YZC6 grew at 10-37 °C, pH 5.5-8.5, and with up to 2 % (w/v) NaCl. The major cellular fatty acids were C and C 11 dimethyl acetal. Phylogenetic analysis of the 16S rRNA gene revealed that strain YZC6 belonged to the genus and was closely related to DSM 5434 (98.3 % sequence similarity), WM1 (98.1 %), and SPL73 (98.1 %). The average nucleotide identity based on blast (below 87.8 %) and digital DNA-DNA hybridization (below 36.1 %) values between the novel isolate and its corresponding relatives showed that strain YZC6 could be readily distinguished from its closely related species. Based on genotypic, phenotypic, and chemotaxonomic data, a novel species, sp. nov., was proposed, with YZC6 as the type strain (=MAFF 212518=JCM 32810=DSM 112100). This study also proposed Gundawar . 2019 as a later heterotypic synonym of (Parshina . 2003) Haas and Blanchard 2020 and Mechichi . 1999 as a later heterotypic synonym of (McClung and McCpy 1957) Haas and Blanchard 2020.
一株革兰氏阴性、产芽孢、杆状、吲哚阳性的细菌菌株,被命名为 YZC6,从发酵的甘蓝中分离得到。菌株 YZC6 在 10-37°C、pH5.5-8.5 和高达 2%(w/v)NaCl 的条件下生长。主要细胞脂肪酸为 C 和 C 11 二甲基缩醛。16S rRNA 基因的系统发育分析表明,菌株 YZC6 属于 属,与 DSM 5434(98.3%序列相似性)、WM1(98.1%)和 SPL73(98.1%)最为密切相关。基于 blast 的平均核苷酸同一性(低于 87.8%)和数字 DNA-DNA 杂交(低于 36.1%)值表明,新型分离株与相关近亲之间的差异较大。根据基因型、表型和化学分类数据,提出了一个新的 种,命名为 sp. nov.,YZC6 为其模式株(=MAFF 212518=JCM 32810=DSM 112100)。本研究还提出了 Gundawar. 2019 是后来的异名,作为 (Parshina. 2003)Haas 和 Blanchard 2020 的同义名,以及 Mechichi. 1999 是后来的异名,作为 (McClung 和 McCpy 1957)Haas 和 Blanchard 2020 的同义名。