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无透镜超薄纤维束尖端的褶痕内镜检查。

Ptycho-endoscopy on a lensless ultrathin fiber bundle tip.

作者信息

Song Pengming, Wang Ruihai, Loetgering Lars, Liu Jia, Vouras Peter, Lee Yujin, Jiang Shaowei, Feng Bin, Maiden Andrew, Yang Changhuei, Zheng Guoan

机构信息

Department of Biomedical Engineering, University of Connecticut, Storrs, CT, 06269, USA.

CarlZeiss AG, Carl Zeiss Promenade, Jena, Thuringia, 07745, Germany.

出版信息

Light Sci Appl. 2024 Jul 17;13(1):168. doi: 10.1038/s41377-024-01510-5.

Abstract

Synthetic aperture radar (SAR) utilizes an aircraft-carried antenna to emit electromagnetic pulses and detect the returning echoes. As the aircraft travels across a designated area, it synthesizes a large virtual aperture to improve image resolution. Inspired by SAR, we introduce synthetic aperture ptycho-endoscopy (SAPE) for micro-endoscopic imaging beyond the diffraction limit. SAPE operates by hand-holding a lensless fiber bundle tip to record coherent diffraction patterns from specimens. The fiber cores at the distal tip modulate the diffracted wavefield within a confined area, emulating the role of the 'airborne antenna' in SAR. The handheld operation introduces positional shifts to the tip, analogous to the aircraft's movement. These shifts facilitate the acquisition of a ptychogram and synthesize a large virtual aperture extending beyond the bundle's physical limit. We mitigate the influences of hand motion and fiber bending through a low-rank spatiotemporal decomposition of the bundle's modulation profile. Our tests demonstrate the ability to resolve a 548-nm linewidth on a resolution target. The achieved space-bandwidth product is ~1.1 million effective pixels, representing a 36-fold increase compared to that of the original fiber bundle. Furthermore, SAPE's refocusing capability enables imaging over an extended depth of field exceeding 2 cm. The aperture synthesizing process in SAPE surpasses the diffraction limit set by the probe's maximum collection angle, opening new opportunities for both fiber-based and distal-chip endoscopy in applications such as medical diagnostics and industrial inspection.

摘要

合成孔径雷达(SAR)利用机载天线发射电磁脉冲并检测回波。当飞机飞越指定区域时,它会合成一个大的虚拟孔径以提高图像分辨率。受SAR启发,我们引入了合成孔径相干衍射内窥镜技术(SAPE),用于超越衍射极限的微观内窥镜成像。SAPE通过手持无透镜纤维束尖端来记录来自标本的相干衍射图案进行操作。远端尖端的纤维芯在有限区域内调制衍射波场,模拟SAR中“机载天线”的作用。手持操作会使尖端产生位置偏移,类似于飞机的移动。这些偏移有助于获取叠层衍射图并合成一个超出纤维束物理极限的大虚拟孔径。我们通过对纤维束调制轮廓进行低秩时空分解来减轻手部运动和纤维弯曲的影响。我们的测试表明能够在分辨率目标上分辨出548纳米的线宽。实现的空间带宽积约为110万个有效像素,与原始纤维束相比增加了36倍。此外,SAPE的重新聚焦能力使其能够在超过2厘米的扩展景深上进行成像。SAPE中的孔径合成过程超越了由探头最大收集角设定的衍射极限,为医学诊断和工业检测等应用中的基于纤维和远端芯片的内窥镜检查开辟了新机遇。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66ce/11255264/fb98f535fc17/41377_2024_1510_Fig1_HTML.jpg

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