Lipovy Bretislav, Vacek Lukas, Kleknerova Dominika Polastik, Jeklova Edita, Liskova Lenka, Holoubek Jakub, Matyskova Dominika, Ruzicka Filip
Department of Burns and Plastic Surgery, Institution Shared with University Hospital Brno, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
Advanced Biomaterials Group, Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic.
Vet Med (Praha). 2024 Jun 27;69(6):191-197. doi: 10.17221/25/2024-VETMED. eCollection 2024 Jun.
poses a significant threat to both immunocompetent and immunocompromised individuals, often resulting in life-threatening infections. With increasing antimicrobial resistance, novel therapeutic strategies are urgently needed. Although animal models are crucial for preclinical studies, limited data are available for porcine models, more specifically for complicated skin and soft tissue infections (cSSTIs). This study presents a novel porcine model inducing and sustaining cSSTI for 14 days. Six pigs (120 wounds) were used for the development of infections, and within this group, two pigs (40 wounds) were used to evaluate the progression of the cSSTI infection. The model demonstrated bacterial loads of more than 10 CFU/gram of tissue or higher. The cSSTI fully developed within three days and remained well above these levels until day 14 post-infection. Due to the immunocompetence of this model, all the immunological processes associated with the response to the presence of infection and the wound healing process are preserved.
对免疫功能正常和免疫功能低下的个体均构成重大威胁，常导致危及生命的感染。随着抗菌药物耐药性的增加，迫切需要新的治疗策略。尽管动物模型对临床前研究至关重要，但关于猪模型的数据有限，更具体地说是关于复杂皮肤和软组织感染（cSSTIs）的数据。本研究提出了一种新的猪模型，可诱导并维持cSSTI 14天。六头猪（120处伤口）用于感染的建立，在该组中，两头猪（40处伤口）用于评估cSSTI感染的进展。该模型显示组织细菌载量超过10 CFU/克或更高。cSSTI在三天内完全形成，并在感染后第14天之前一直远高于这些水平。由于该模型具有免疫能力，与感染反应和伤口愈合过程相关的所有免疫过程均得以保留。
