• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

转录组和微生物组-免疫在癌前和侵袭性肛门癌病变中的变化。

Transcriptome and microbiome-immune changes across preinvasive and invasive anal cancer lesions.

机构信息

Centro de Investigaciones Inmunológicas Básicas y Aplicadas (CINIBA), Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata, Argentina.

University of Miami - Center for AIDS Research/Sylvester Cancer Comprehensive Center Argentina Consortium for Research and Training in Virally Induced AIDS-Malignancies, University of Miami Miller School of Medicine, Miami, Florida, USA (detailed in Supplemental Acknowledgments).

出版信息

JCI Insight. 2024 Jul 18;9(16):e180907. doi: 10.1172/jci.insight.180907.

DOI:10.1172/jci.insight.180907
PMID:39024554
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11343604/
Abstract

Anal squamous cell carcinoma (ASCC) is a rare gastrointestinal malignancy linked to high-risk human papillomavirus (HPV) infection, which develops from precursor lesions like low-grade squamous intraepithelial lesions and high-grade squamous intraepithelial lesions (HGSILs). ASCC incidence varies across populations and poses increased risk for people living with HIV. Our investigation focused on transcriptomic and metatranscriptomic changes from squamous intraepithelial lesions to ASCC. Metatranscriptomic analysis highlighted specific bacterial species (e.g., Fusobacterium nucleatum, Bacteroides fragilis) more prevalent in ASCC than precancerous lesions. These species correlated with gene-encoding enzymes (Acca, glyQ, eno, pgk, por) and oncoproteins (FadA, dnaK), presenting potential diagnostic or treatment markers. Unsupervised transcriptomic analysis identified distinct sample clusters reflecting histological diagnosis, immune infiltrate, HIV/HPV status, and pathway activities, recapitulating anal cancer progression's natural history. Our study unveiled molecular mechanisms in anal cancer progression, aiding in stratifying HGSIL cases based on low or high risk of progression to malignancy.

摘要

肛门鳞状细胞癌 (ASCC) 是一种罕见的胃肠道恶性肿瘤，与高危型人乳头瘤病毒 (HPV) 感染有关，它由低级别鳞状上皮内病变和高级别鳞状上皮内病变 (HGSIL) 等前驱病变发展而来。ASCC 的发病率在不同人群中存在差异，且 HIV 感染者的患病风险增加。我们的研究重点是从鳞状上皮内病变到 ASCC 的转录组和宏转录组变化。宏转录组分析突出了特定的细菌物种（例如，具核梭杆菌、脆弱拟杆菌）在 ASCC 中比癌前病变更为普遍。这些物种与编码酶（Acca、glyQ、eno、pgk、por）和致癌蛋白（FadA、dnaK）相关，具有潜在的诊断或治疗标志物。无监督转录组分析确定了不同的样本簇，反映了组织学诊断、免疫浸润、HIV/HPV 状态和途径活性，再现了肛门癌进展的自然史。我们的研究揭示了肛门癌进展中的分子机制，有助于根据向恶性肿瘤进展的低风险或高风险对 HGSIL 病例进行分层。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/788e/11343604/69050c597095/jciinsight-9-180907-g031.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/788e/11343604/13b84ff31c3b/jciinsight-9-180907-g023.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/788e/11343604/cff4df54cd75/jciinsight-9-180907-g024.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/788e/11343604/d5d83c55cab2/jciinsight-9-180907-g025.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/788e/11343604/61d00e19f454/jciinsight-9-180907-g026.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/788e/11343604/de39c1b1d376/jciinsight-9-180907-g027.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/788e/11343604/e0d8e5214526/jciinsight-9-180907-g028.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/788e/11343604/3a09cb2b383e/jciinsight-9-180907-g029.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/788e/11343604/ad6013f3805b/jciinsight-9-180907-g030.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/788e/11343604/69050c597095/jciinsight-9-180907-g031.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/788e/11343604/13b84ff31c3b/jciinsight-9-180907-g023.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/788e/11343604/cff4df54cd75/jciinsight-9-180907-g024.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/788e/11343604/d5d83c55cab2/jciinsight-9-180907-g025.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/788e/11343604/61d00e19f454/jciinsight-9-180907-g026.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/788e/11343604/de39c1b1d376/jciinsight-9-180907-g027.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/788e/11343604/e0d8e5214526/jciinsight-9-180907-g028.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/788e/11343604/3a09cb2b383e/jciinsight-9-180907-g029.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/788e/11343604/ad6013f3805b/jciinsight-9-180907-g030.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/788e/11343604/69050c597095/jciinsight-9-180907-g031.jpg

相似文献

1
Transcriptome and microbiome-immune changes across preinvasive and invasive anal cancer lesions.转录组和微生物组-免疫在癌前和侵袭性肛门癌病变中的变化。
JCI Insight. 2024 Jul 18;9(16):e180907. doi: 10.1172/jci.insight.180907.
2
HIV-Infected Patients With Anal Cancer Precursors: Clinicopathological Characteristics and Human Papillomavirus Subtype Distribution.人类免疫缺陷病毒感染患者的肛门癌前病变：临床病理特征和人乳头瘤病毒亚型分布。
Dis Colon Rectum. 2020 Jul;63(7):890-896. doi: 10.1097/DCR.0000000000001671.
3
Natural History of Anal Squamous Intraepithelial Lesions in HIV-Positive Men with Normal Baseline Cytology.HIV 阳性男性中基线细胞学正常的肛门鳞状上皮内病变自然史。
AIDS Patient Care STDS. 2019 Nov;33(11):459-465. doi: 10.1089/apc.2019.0186.
4
Prevalence of abnormal anal cytology and high-grade squamous intraepithelial lesions among a cohort of HIV-infected men who have sex with men.HIV 感染的男男性行为人群中异常肛门细胞学和高级别鳞状上皮内病变的流行率。
Dis Colon Rectum. 2014 Apr;57(4):475-81. doi: 10.1097/DCR.0000000000000095.
5
Presence of high-risk human papillomavirus genotype and human immunodeficiency virus DNA in anal high-grade and low-grade squamous intraepithelial lesions.肛门高级别和低级别鳞状上皮内病变中高危型人乳头瘤病毒基因型和人类免疫缺陷病毒DNA的存在情况。
AIDS Res Hum Retroviruses. 2013 Jan;29(1):178-81. doi: 10.1089/AID.2012.0136. Epub 2012 Aug 27.
6
Anal Cancer Precursor Lesions in HIV-Infected Persons: Tissue Human Papillomavirus Type Distribution and Impact on Treatment Response.人类免疫缺陷病毒感染人群中的肛门癌前病变：组织人乳头瘤病毒型别分布及其对治疗反应的影响。
Dis Colon Rectum. 2019 May;62(5):579-585. doi: 10.1097/DCR.0000000000001307.
7
Anal dysplasia and anal cancer screening practices among liver transplant centers in the United States: Results of an online survey.美国肝脏移植中心的肛门发育不良和肛门癌筛查实践：一项在线调查的结果。
Transpl Infect Dis. 2024 Aug;26(4):e14286. doi: 10.1111/tid.14286. Epub 2024 May 2.
8
Expression of microRNAs 16, 20a, 150 and 155 in anal squamous intraepithelial lesions from high-risk groups.高危人群肛门鳞状上皮内病变中 microRNAs 16、20a、150 和 155 的表达。
Sci Rep. 2019 Feb 6;9(1):1523. doi: 10.1038/s41598-018-38378-6.
9
Host Nuclear Genome Copy Number Variations Identify High-Risk Anal Precancers in People Living With HIV.宿主核基因组拷贝数变异可识别艾滋病毒感染者的高危肛门前癌。
J Acquir Immune Defic Syndr. 2024 Jun 1;96(2):190-195. doi: 10.1097/QAI.0000000000003409.
10
Methylation markers for anal cancer screening: A repeated cross-sectional analysis of people living with HIV, 2015-2016.肛门癌筛查的甲基化标志物：2015-2016 年 HIV 感染者的重复横断面分析。
Int J Cancer. 2024 Sep 15;155(6):1091-1100. doi: 10.1002/ijc.34977. Epub 2024 Apr 29.

引用本文的文献

1
Human Papillomavirus-Encoded microRNAs as Regulators of Human Gene Expression in Anal Squamous Cell Carcinoma: A Meta-Transcriptomics Study.人乳头瘤病毒编码的微小RNA作为肛门鳞状细胞癌中人类基因表达的调节因子：一项元转录组学研究
Noncoding RNA. 2025 Jun 9;11(3):43. doi: 10.3390/ncrna11030043.

本文引用的文献

1
Microbiome-derived cobalamin and succinyl-CoA as biomarkers for improved screening of anal cancer.微生物组衍生的钴胺素和琥珀酰辅酶 A 作为提高肛门癌筛查的生物标志物。
Nat Med. 2023 Jul;29(7):1738-1749. doi: 10.1038/s41591-023-02407-3. Epub 2023 Jul 18.
2
Oral and anal microbiome from HIV-exposed individuals: role of host-associated factors in taxa composition and metabolic pathways.HIV 暴露个体的口腔和肛门微生物组：宿主相关因素对分类群组成和代谢途径的作用。
NPJ Biofilms Microbiomes. 2023 Jul 12;9(1):48. doi: 10.1038/s41522-023-00413-4.
3
Relationship between Human Papillomavirus Status and the Cervicovaginal Microbiome in Cervical Cancer.
宫颈癌中人乳头瘤病毒状态与宫颈阴道微生物群的关系
Microorganisms. 2023 May 27;11(6):1417. doi: 10.3390/microorganisms11061417.
4
Immunotherapy in Anal Cancer.肛门癌的免疫治疗。
Curr Oncol. 2023 Apr 27;30(5):4538-4550. doi: 10.3390/curroncol30050343.
5
HPV-related anal cancer is associated with changes in the anorectal microbiome during cancer development.HPV 相关的肛门癌与癌症发展过程中肛门直肠微生物组的变化有关。
Front Immunol. 2023 Mar 29;14:1051431. doi: 10.3389/fimmu.2023.1051431. eCollection 2023.
6
HPV16 E6 induces chromosomal instability due to polar chromosomes caused by E6AP-dependent degradation of the mitotic kinesin CENP-E.HPV16 E6 通过 E6AP 依赖性降解有丝分裂运动蛋白 CENP-E 导致极性染色体，从而引起染色体不稳定。
Proc Natl Acad Sci U S A. 2023 Apr 4;120(14):e2216700120. doi: 10.1073/pnas.2216700120. Epub 2023 Mar 29.
7
Autologous T cell therapy for MAGE-A4 solid cancers in HLA-A*02 patients: a phase 1 trial.自体 T 细胞疗法治疗 HLA-A*02 患者的 MAGE-A4 实体瘤：一项 1 期临床试验。
Nat Med. 2023 Jan;29(1):104-114. doi: 10.1038/s41591-022-02128-z. Epub 2023 Jan 9.
8
The Role of HERV-K in Cancer Stemness.HERV-K 在癌症干性中的作用。
Viruses. 2022 Sep 12;14(9):2019. doi: 10.3390/v14092019.
9
Genital Microbiota of Women From Six Ethnic Groups With and Without Human Papillomavirus Infection in Shangri-La, China.中国香格里拉六个人群中 HPV 感染和未感染女性的生殖道微生物组。
Front Cell Infect Microbiol. 2022 Jul 8;12:935068. doi: 10.3389/fcimb.2022.935068. eCollection 2022.
10
Microbiome Dynamics During Chemoradiation Therapy for Anal Cancer.分析癌症放化疗过程中的微生物组动态变化。
Int J Radiat Oncol Biol Phys. 2022 Aug 1;113(5):974-984. doi: 10.1016/j.ijrobp.2022.04.037. Epub 2022 May 2.