Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, China.
Gynaecology Department, The First People's Hospital of Yunnan Province, Kunming, China.
Front Cell Infect Microbiol. 2022 Jul 8;12:935068. doi: 10.3389/fcimb.2022.935068. eCollection 2022.
A diversity of microorganisms is associated with human health and exists in a state of dynamic equilibrium. This diversity has direct implications for the assessment of susceptibility to infectious diseases, especially human papillomavirus (HPV) infection.
Here, we investigated the relationships between HPV infection and vaginal, cervical, and gut microbiota composition and assessed the levels of genital immune mediators. We selected a multiethnic area in Yunnan Province, China, to collect samples from healthy women of childbearing age. A total of 82 healthy women of childbearing age were included in this study. Vaginal, cervical, and rectal swabs were collected to analyze the microbial community, and cytokines were analyzed in some samples.
Different proportions and types of HPV infection were detected in cervical (44%), vaginal (18%), and rectal (18%) swabs. HPV detected in cervical swabs was generally a high-risk type, while low-risk HPV types were primarily detected in vaginal and rectal swabs. There were some differences in this proportion as well as in the microbial community composition among different ethnic groups. Rectal samples exhibited the highest diversity index, while vaginal samples displayed the lowest diversity index. dominated most of the vaginal samples, was decreased in HPV-positive samples, and differed among different ethnic groups. However, the sequence proportion of in the cervix exhibited the opposite trend in those affected by HPV infection. The dynamic balance between the potential pathogens and determines the health of the female genital system.
This study constitutes the first step toward personalized medicine for women's reproductive health, wherein differences between the genital microbiomes of individuals would be considered in risk assessment and for subsequent disease diagnosis and treatment.
多种微生物与人类健康相关，并处于动态平衡状态。这种多样性直接影响到对传染病易感性的评估，尤其是人乳头瘤病毒（HPV）感染。
本研究旨在探讨 HPV 感染与阴道、宫颈和肠道微生物群落组成之间的关系，并评估生殖道免疫介质的水平。我们选择了中国云南省的一个多民族地区，收集了育龄健康女性的样本。本研究共纳入 82 名育龄健康女性。采集阴道、宫颈和直肠拭子进行微生物群落分析，并对部分样本进行细胞因子分析。
宫颈（44%）、阴道（18%）和直肠（18%）拭子中均检测到不同比例和类型的 HPV 感染。宫颈拭子中 HPV 多为高危型，而阴道和直肠拭子中主要检测到低危 HPV 型。不同种族之间，这种比例以及微生物群落组成存在差异。直肠样本的多样性指数最高，而阴道样本的多样性指数最低。 是阴道样本中的优势菌，在 HPV 阳性样本中减少，且在不同种族之间存在差异。然而，HPV 感染对宫颈部位的 序列比例产生了相反的影响。潜在病原体 和 之间的动态平衡决定了女性生殖道的健康状况。
本研究是迈向女性生殖健康个体化医学的第一步，其中个体生殖道微生物组的差异将在风险评估以及随后的疾病诊断和治疗中得到考虑。
