Morrison D P, Hastings P J
Mol Gen Genet. 1979 Aug;175(1):57-65. doi: 10.1007/BF00267856.
The mutator mutation mut5-1 has been characterized with respect to a range of parameters which have been used to describe DNA repair mutants of yeast. No marked effect of the mutation on UV-mutability at lower doses was apparent. Diploids homozygous for the mutation are deficient in UV-induced recombination between the alleles his1-1 and hist1-315, mutation being sufficient to account for all the UV-induced histidine prototrophs. Complementation and mapping studies indicate that mut5-1 is allelic to rad51-1, supporting the conclusion of Hastings et al. (1976) that a mutator may increase spontaneous mutation by modifying repair parameters. Both mut5-1 homozygous and heterozygous diploids give rise to spontaneous or UV-induced segregants which appear to be the products of nondisjunction events. The levels of parameiotic recombination (see Sherman and Roman, 1963; Esposito and Esposito, 1974), sporulation and spore viability observed in mut5-1/mut5-1 diploids indicate that the function encoded by RAD51 is required at 2 times during meiosis. An essential role of the function encoded by RAD51 in mitotic and meiotic recombination is indicated.
已针对一系列用于描述酵母DNA修复突变体的参数对突变体mut5-1进行了表征。在较低剂量下，该突变对紫外线诱变没有明显影响。该突变的纯合二倍体在等位基因his1-1和hist1-315之间的紫外线诱导重组方面存在缺陷，该突变足以解释所有紫外线诱导的组氨酸原养型。互补和定位研究表明，mut5-1与rad51-1等位，支持了黑斯廷斯等人（1976年）的结论，即突变体可能通过改变修复参数来增加自发突变。mut5-1纯合和杂合二倍体都会产生自发的或紫外线诱导的分离子，这些分离子似乎是非分离事件的产物。在mut5-1/mut5-1二倍体中观察到的减数分裂前重组水平（见谢尔曼和罗曼，1963年；埃斯波西托和埃斯波西托，1974年）、孢子形成和孢子活力表明，RAD51编码的功能在减数分裂过程中需要两次。这表明RAD51编码的功能在有丝分裂和减数分裂重组中起着重要作用。
