• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

细胞外通量分析（ Seahorse 分析）：在跨生物学科的代谢研究中的多种应用。

Extracellular flux assay (Seahorse assay): Diverse applications in metabolic research across biological disciplines.

机构信息

Department of Microbiology and Biotechnology, Dankook University, Cheonan, Republic of Korea.

Department of Biomedical Science & Systems Biology, Dankook University, Cheonan, Republic of Korea.

出版信息

Mol Cells. 2024 Aug;47(8):100095. doi: 10.1016/j.mocell.2024.100095. Epub 2024 Jul 18.

DOI:10.1016/j.mocell.2024.100095
PMID:39032561
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11374971/
Abstract

Metabolic networks are fundamental to cellular processes, driving energy production, biosynthesis, redox regulation, and cellular signaling. Recent advancements in metabolic research tools have provided unprecedented insights into cellular metabolism. Among these tools, the extracellular flux analyzer stands out for its real-time measurement of key metabolic parameters: glycolysis, mitochondrial respiration, and fatty acid oxidation, leading to its widespread use. This review provides a comprehensive summary of the basic principles and workflow of the extracellular flux assay (the Seahorse assay) and its diverse applications. We highlight the assay's versatility across various biological models, including cancer cells, immunocytes, Caenorhabditis elegans, tissues, isolated mitochondria, and three-dimensional structures such as organoids, and summarize key considerations for using extracellular flux assay in these models. Additionally, we discuss the limitations of the Seahorse assay and propose future directions for its development. This review aims to enhance the understanding of extracellular flux assay and its significance in biological studies.

摘要

代谢网络是细胞过程的基础，驱动着能量产生、生物合成、氧化还原调节和细胞信号转导。代谢研究工具的最新进展为细胞代谢提供了前所未有的见解。在这些工具中，由于能够实时测量糖酵解、线粒体呼吸和脂肪酸氧化等关键代谢参数，外切体流量分析仪脱颖而出，得到了广泛应用。本综述全面总结了外切体流量分析（ Seahorse 分析）的基本原理和工作流程及其多样化的应用。我们强调了该分析在包括癌细胞、免疫细胞、秀丽隐杆线虫、组织、分离的线粒体以及类器官等多种生物模型中的多功能性，并总结了在这些模型中使用外切体流量分析的关键注意事项。此外，我们还讨论了 Seahorse 分析的局限性，并提出了该分析的未来发展方向。本综述旨在提高对外切体流量分析及其在生物学研究中的重要性的理解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57f2/11374971/934161707e3a/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57f2/11374971/58753ed567be/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57f2/11374971/db38c571a969/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57f2/11374971/f72f262cbbf0/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57f2/11374971/934161707e3a/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57f2/11374971/58753ed567be/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57f2/11374971/db38c571a969/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57f2/11374971/f72f262cbbf0/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57f2/11374971/934161707e3a/gr4.jpg

相似文献

1
Extracellular flux assay (Seahorse assay): Diverse applications in metabolic research across biological disciplines.细胞外通量分析（ Seahorse 分析）：在跨生物学科的代谢研究中的多种应用。
Mol Cells. 2024 Aug;47(8):100095. doi: 10.1016/j.mocell.2024.100095. Epub 2024 Jul 18.
2
Studying the Metabolism of Epithelial-Mesenchymal Plasticity Using the Seahorse XFe96 Extracellular Flux Analyzer.使用 Seahorse XFe96 细胞外通量分析仪研究上皮-间充质可塑性的代谢。
Methods Mol Biol. 2021;2179:327-340. doi: 10.1007/978-1-0716-0779-4_25.
3
Seahorse Xfe 24 Extracellular Flux Analyzer-Based Analysis of Cellular Respiration in Caenorhabditis elegans.基于海马Xfe 24细胞外流量分析仪对秀丽隐杆线虫细胞呼吸的分析
Curr Protoc Toxicol. 2015 Nov 2;66:25.7.1-25.7.15. doi: 10.1002/0471140856.tx2507s66.
4
Analyzing Melanoma Cell Oxygen Consumption and Extracellular Acidification Rates Using Seahorse Technology.利用 Seahorse 技术分析黑素瘤细胞耗氧量和细胞外酸化率。
Methods Mol Biol. 2021;2265:81-89. doi: 10.1007/978-1-0716-1205-7_6.
5
Metabolic analysis of mouse bone-marrow-derived dendritic cells using an extracellular flux analyzer.采用细胞外通量分析仪对小鼠骨髓来源树突状细胞进行代谢分析。
STAR Protoc. 2021 Mar 26;2(2):100401. doi: 10.1016/j.xpro.2021.100401. eCollection 2021 Jun 18.
6
Bioenergetic analysis of intact mammalian cells using the Seahorse XF24 Extracellular Flux analyzer and a luciferase ATP assay.使用海马XF24细胞外通量分析仪和荧光素酶ATP检测法对完整哺乳动物细胞进行生物能量分析。
Methods Mol Biol. 2014;1105:589-602. doi: 10.1007/978-1-62703-739-6_40.
7
Extracellular Flux Assays to Determine Oxidative Phosphorylation and Glycolysis in Chronic Lymphocytic Leukemia Cells.用于测定慢性淋巴细胞白血病细胞中氧化磷酸化和糖酵解的细胞外通量分析
Methods Mol Biol. 2019;1881:121-128. doi: 10.1007/978-1-4939-8876-1_10.
8
Measurement of mitochondrial respiration in the murine retina using a Seahorse extracellular flux analyzer.使用 Seahorse 细胞外通量分析仪测量鼠视网膜中的线粒体呼吸。
STAR Protoc. 2021 May 12;2(2):100533. doi: 10.1016/j.xpro.2021.100533. eCollection 2021 Jun 18.
9
Live Metabolic Profile Analysis of Zebrafish Embryos Using a Seahorse XF 24 Extracellular Flux Analyzer.使用海马XF 24细胞外通量分析仪对斑马鱼胚胎进行实时代谢谱分析。
Methods Mol Biol. 2018;1797:393-401. doi: 10.1007/978-1-4939-7883-0_21.
10
Real-Time Assessment of Mitochondrial Function in Cytotrophoblast and Syncytialized Trophoblast Cells Using the Seahorse XFe24 Extracellular Flux Analyzer.使用 Seahorse XFe24 细胞外通量分析仪实时评估滋养细胞和合体滋养层细胞中的线粒体功能。
Methods Mol Biol. 2024;2728:137-147. doi: 10.1007/978-1-0716-3495-0_12.

引用本文的文献

1
Protocol to assess changes in mitochondrial respiration in living cultured cells infected with flaviviruses.评估感染黄病毒的活培养细胞中线粒体呼吸变化的实验方案。
STAR Protoc. 2025 Aug 28;6(3):104051. doi: 10.1016/j.xpro.2025.104051.
2
Metabolic Disturbances Involved in Cardiovascular Diseases: The Role of Mitochondrial Dysfunction, Altered Bioenergetics and Oxidative Stress.心血管疾病中的代谢紊乱：线粒体功能障碍、生物能量改变及氧化应激的作用
Int J Mol Sci. 2025 Jul 15;26(14):6791. doi: 10.3390/ijms26146791.
3
NEXN deficiency leads to dilated cardiomyopathy in human pluripotent stem cell-derived cardiomyocytes.

本文引用的文献

1
Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of inflammatory pain.神经元-星形胶质细胞代谢偶联促进脊髓可塑性和炎症性疼痛的维持。
Nat Metab. 2024 Mar;6(3):494-513. doi: 10.1038/s42255-024-01001-2. Epub 2024 Mar 5.
2
Obesity causes mitochondrial fragmentation and dysfunction in white adipocytes due to RalA activation.肥胖症会导致白色脂肪细胞中线粒体的碎片化和功能障碍，这是由于 RalA 的激活。
Nat Metab. 2024 Feb;6(2):273-289. doi: 10.1038/s42255-024-00978-0. Epub 2024 Jan 29.
3
Disruption of lysosomal proteolysis in astrocytes facilitates midbrain organoid proteostasis failure in an early-onset Parkinson's disease model.
NEXN 缺乏会导致人类多能干细胞衍生的心肌细胞出现扩张型心肌病。
Stem Cell Res Ther. 2025 Jul 26;16(1):402. doi: 10.1186/s13287-025-04484-2.
4
Decoding sex differences in human immunity through systems immunology.通过系统免疫学解析人类免疫中的性别差异。
Oxf Open Immunol. 2025 Jul 4;6(1):iqaf006. doi: 10.1093/oxfimm/iqaf006. eCollection 2025.
5
ATP synthase inhibition, an overlooked confounding factor in the mitochondrial stress test.ATP合酶抑制，线粒体应激试验中一个被忽视的混杂因素。
PLoS One. 2025 Jul 17;20(7):e0328256. doi: 10.1371/journal.pone.0328256. eCollection 2025.
6
Intracellular Formation of Synthetic Peptide Nanostructures Causes Mitochondrial Disruption and Cell Death in Tumor Spheroids.合成肽纳米结构的细胞内形成导致肿瘤球体中的线粒体破坏和细胞死亡。
Adv Sci (Weinh). 2025 Jul;12(25):e2412606. doi: 10.1002/advs.202412606. Epub 2025 May 26.
7
senescence and senolytic functional assays.衰老和衰老细胞溶解功能测定
Biomater Sci. 2025 Jun 25;13(13):3509-3531. doi: 10.1039/d4bm01684j.
8
Measurement of oxygen consumption rate in mouse aortic tissue.小鼠主动脉组织氧消耗率的测量。
Biol Methods Protoc. 2025 Apr 24;10(1):bpaf031. doi: 10.1093/biomethods/bpaf031. eCollection 2025.
9
Astragaloside IV mitigates influenza-induced inflammatory responses by suppressing the Wnt/β-catenin signalling pathway in alveolar macrophages.黄芪甲苷通过抑制肺泡巨噬细胞中的Wnt/β-连环蛋白信号通路减轻流感诱导的炎症反应。
Vet Res. 2025 Apr 30;56(1):95. doi: 10.1186/s13567-025-01529-5.
10
Supinoxin blocks small cell lung cancer progression by inhibiting mitochondrial respiration through DDX5.舒匹诺辛通过DDX5抑制线粒体呼吸来阻断小细胞肺癌的进展。
iScience. 2025 Mar 13;28(4):112219. doi: 10.1016/j.isci.2025.112219. eCollection 2025 Apr 18.
星形胶质细胞溶酶体蛋白水解的破坏促进早发性帕金森病模型中中脑细胞体的蛋白稳态失调。
Nat Commun. 2024 Jan 10;15(1):447. doi: 10.1038/s41467-024-44732-2.
4
Endogenous renal adiponectin drives gluconeogenesis through enhancing pyruvate and fatty acid utilization.内源性肾脂联素通过增强丙酮酸和脂肪酸利用来驱动糖异生。
Nat Commun. 2023 Oct 17;14(1):6531. doi: 10.1038/s41467-023-42188-4.
5
The eIF4EBP-eIF4E axis regulates CD4 T cell differentiation through modulation of T cell activation and metabolism.真核细胞起始因子4E结合蛋白（eIF4EBP）-真核细胞起始因子4E（eIF4E）轴通过调节T细胞活化和代谢来调控CD4 T细胞分化。
iScience. 2023 Apr 18;26(5):106683. doi: 10.1016/j.isci.2023.106683. eCollection 2023 May 19.
6
Implications of hydrogen sulfide in colorectal cancer: Mechanistic insights and diagnostic and therapeutic strategies.硫化氢在结直肠癌中的作用：机制见解及诊断与治疗策略。
Redox Biol. 2023 Feb;59:102601. doi: 10.1016/j.redox.2023.102601. Epub 2023 Jan 7.
7
Sex differences in heart mitochondria regulate diastolic dysfunction.心脏线粒体中的性别差异调节舒张功能障碍。
Nat Commun. 2022 Jul 4;13(1):3850. doi: 10.1038/s41467-022-31544-5.
8
The Use of Seahorse XF Assays to Interrogate Real-Time Energy Metabolism in Cancer Cell Lines.使用 Seahorse XF 检测分析实时检测癌细胞系的能量代谢
Methods Mol Biol. 2022;2508:225-234. doi: 10.1007/978-1-0716-2376-3_17.
9
Inhibition of the succinyl dehydrogenase complex in acute myeloid leukemia leads to a lactate-fuelled respiratory metabolic vulnerability.急性髓系白血病中琥珀酰脱氢酶复合物的抑制导致以乳酸为燃料的呼吸代谢脆弱性。
Nat Commun. 2022 Apr 19;13(1):2013. doi: 10.1038/s41467-022-29639-0.
10
Positive feedback regulation of microglial glucose metabolism by histone H4 lysine 12 lactylation in Alzheimer's disease.阿尔茨海默病中组蛋白 H4 赖氨酸 12 乳酰化对小胶质细胞葡萄糖代谢的正反馈调节。
Cell Metab. 2022 Apr 5;34(4):634-648.e6. doi: 10.1016/j.cmet.2022.02.013. Epub 2022 Mar 17.