Obsa Terfa Temesgen, Abera Kebede Fekadu, Kitessa Beyene Monas, Tesfaye Abebe Tilahun, Basazn Mekuria Abebe, Seid Yesuf Jibril
Department of Medicine College of Medicine and Health Sciences Jigjiga University, P. O. Box 1020, Jigjiga, Ethiopia.
Department of Nursing College of Health Sciences Oda Bultum University, Chiro, Ethiopia.
Evid Based Complement Alternat Med. 2024 Jul 12;2024:2728282. doi: 10.1155/2024/2728282. eCollection 2024.
Diarrhea is the common gastrointestinal disorder accounting for 2.5 billion episodes and 1.5 million deaths annually. Limitations and inaccessibility of currently available medications are the main problem associated with treatment of diarrhea. Hence, medicinal plants are usually preferred to manage diarrhea because they may contain constituents with high activity and fewer side effects. Even though the dose, safety, and efficacy of (L.) Schott are not substantiated scientifically, several societies use it for the treatment of diarrhea.
This study was targeted at exploring the in vivo antidiarrheal activities of 80% methanol extract and solvent fractions of peels of (L.) Schott in Swiss albino mice.
The plant was collected and extracted with 80% methanol, followed by fractionation with distilled water, chloroform, and diethyl ether. Castor oil-induced diarrhea, enteropooling, and motility tests were used to evaluate antidiarrheal activity. The test groups received graded doses of 100 mg/kg, 200 mg/kg, and 400 mg/kg. Negative controls received 10 ml/kg of 2% Tween 80 while positive controls received loperamide (3 mg/kg) or atropine (5 mg/kg).
The crude and solvent fractions of the plant extract have induced significant effects in reduction of the number and weight of wet stools at all tested doses. However, delay in onset of diarrhea was observed only at 400 mg/kg ( < 0.001) for both crude extract and solvent fractions. In antienteropooling test, 80% methanol extract and solvent fractions have significantly reduced the weight and volume of intestinal contents, especially at 200 mg/kg and 400 mg/kg. Regarding the antimotility test, the crude extract reduced motility at all tested doses, whereas the solvent fractions reduced intestinal motility mainly at 400 mg/kg ( < 0.001).
The study has revealed that the 80% methanol extract and solvent fractions of the plant possess antidiarrheal activities supporting the traditional antidiarrheal claims of the plant.
腹泻是常见的胃肠道疾病，每年发生25亿例，导致150万人死亡。目前可用药物的局限性和不可及性是腹泻治疗相关的主要问题。因此，药用植物通常更受青睐用于治疗腹泻，因为它们可能含有活性高且副作用少的成分。尽管肖特氏[植物名称]的剂量、安全性和疗效尚未得到科学证实，但一些群体仍用其治疗腹泻。
本研究旨在探究肖特氏[植物名称]果皮80%甲醇提取物及其溶剂萃取物在瑞士白化小鼠体内的抗腹泻活性。
采集该植物并用80%甲醇提取，随后用蒸馏水、氯仿和乙醚进行萃取。采用蓖麻油诱导腹泻、肠积液和动力试验来评估抗腹泻活性。试验组接受100mg/kg、200mg/kg和400mg/kg的分级剂量。阴性对照组接受10ml/kg的2%吐温80，而阳性对照组接受洛哌丁胺（3mg/kg）或阿托品（5mg/kg）。
植物提取物的粗提物和溶剂萃取物在所有测试剂量下均对减少湿粪的数量和重量产生显著效果。然而，仅在400mg/kg剂量时（P<0.001），粗提物和溶剂萃取物均观察到腹泻发作延迟。在抗肠积液试验中，80%甲醇提取物和溶剂萃取物显著降低了肠内容物的重量和体积，尤其是在200mg/kg和400mg/kg剂量时。关于抗动力试验，粗提物在所有测试剂量下均降低了动力，而溶剂萃取物主要在400mg/kg剂量时降低肠道动力（P<0.001）。
该研究表明，该植物的80%甲醇提取物及其溶剂萃取物具有抗腹泻活性，支持了该植物传统的抗腹泻说法。
