Momose Yoshika, Sasaki Yoshimasa, Yonemitsu Kenzo, Kuroda Makoto, Ikeda Tetsuya, Uema Masashi, Furuya Yoko, Toyofuku Hajime, Igimi Shizunobu, Asai Tetsuo
Division of Biomedical Food Research, National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-9501, Japan.
Division of Veterinary Science, Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.
Food Saf (Tokyo). 2024 May 31;12(2):25-33. doi: 10.14252/foodsafetyfscj.D-24-00001. eCollection 2024 Jun.
infections represent a leading cause of foodborne illnesses; resistance to third-generation cephalosporins (TGCs), which are a first-choice antimicrobial for treating human enteritis, has become a serious public health concern worldwide. Because the consumption of undercooked chicken meat products is a major cause of foodborne salmonellosis in Japan, we conducted three surveys at different periods between 2017 and 2022, with the cooperation of four abattoirs (two in Eastern and two in Western Japan). The first survey was conducted at abattoir A, which is located in Eastern Japan. was detected in 84.4% of broiler flocks tested (27/32); among them, all the TGC-resistant isolates obtained from one farm (farm FA) were identified as Infantis. was recovered from 62.5% of breast meat samples (20/32), with one case suggesting cross-contamination. The second survey was conducted at three other abattoirs to examine the prevalence of TGC-resistant , in both Western (abattoirs B and C) and Eastern (abattoir D) Japan. was detected in 90.6% of broiler flocks examined (29/32). TGC-resistant Infantis was isolated from 2 flocks until 2018 and not thereafter. Subsequently, isolates were identified as TGC-susceptible Schwarzengrund in both regions. The third survey was performed at abattoir A to elucidate whether there were changes in the phenotypes. Of the 11 broiler flocks introduced from farm FA, 10 were positive for (90.9%); all the isolates were Schwarzengrund susceptible to TGC. This study shows that TGC-susceptible Schwarzengrund has replaced the resistant phenotypes among broiler flocks in both Eastern and Western Japan. Although chicken meat products could be cross-contaminated with during the slaughtering process, reducing the prevalence of in broiler flocks remains important to decrease enteritis in humans.
感染是食源性疾病的主要原因之一；对第三代头孢菌素（TGCs）产生耐药性，而第三代头孢菌素是治疗人类肠炎的首选抗菌药物，这已成为全球严重的公共卫生问题。由于食用未煮熟的鸡肉制品是日本食源性沙门氏菌病的主要原因，我们在2017年至2022年期间的不同时间段，在四家屠宰场（日本东部两家，西部两家）的合作下进行了三项调查。第一次调查在位于日本东部的屠宰场A进行。在检测的84.4%的肉鸡群（27/32）中检测到了[具体细菌名称未给出]；其中，从一个农场（农场FA）获得的所有耐TGC分离株均被鉴定为婴儿亚种。从62.5%的胸肉样本（20/32）中分离出了[具体细菌名称未给出]，有一例表明存在交叉污染。第二次调查在其他三家屠宰场进行，以检查日本西部（屠宰场B和C）和东部（屠宰场D）耐TGC[具体细菌名称未给出]的流行情况。在检查的90.6%的肉鸡群（29/32）中检测到了[具体细菌名称未给出]。直到2018年，从2个鸡群中分离出了耐TGC的婴儿亚种，此后未再分离出。随后，两个地区的分离株均被鉴定为对TGC敏感的施瓦岑格鲁德亚种。第三次调查在屠宰场A进行，以阐明表型是否有变化。从农场FA引入的11个肉鸡群中，10个呈阳性（90.9%）；所有分离株均为对TGC敏感的施瓦岑格鲁德亚种。这项研究表明，在日本东部和西部的肉鸡群中，对TGC敏感的施瓦岑格鲁德亚种已取代了耐药表型。尽管鸡肉制品在屠宰过程中可能会被[具体细菌名称未给出]交叉污染，但降低肉鸡群中[具体细菌名称未给出]的流行率对于减少人类的[具体细菌名称未给出]肠炎仍然很重要。
