Key Laboratory of Aquatic Products Processing of Zhejiang Province, Institute of Seafood, Zhejiang Gongshang University, Hangzhou 310018, China.
School of Pharmacy, Hangzhou Normal University, Hangzhou, Zhejiang 311121, China.
Bioresour Technol. 2024 Sep;407:131142. doi: 10.1016/j.biortech.2024.131142. Epub 2024 Jul 21.
In this study, a sustainable and environmentally friendly method was developed for the enrichment and purification of phycocyanin from Spirulina platensis. This was achieved by utilizing a temperature-sensitive polymer, Pluronic F68, in an aqueous two-phase solvent system. The phase behavior of the temperature-sensitive polymer-based biphasic system was evaluated. The extraction conditions were optimized by both single-factor experiments and response surface methodology. Under the optimal conditions, the upper polymer-rich phase was recycled for sustainable phycocyanin extraction, resulting in a grade of 3.23 during the third extraction cycle. Pluronic F68 could be efficiently recovered and reused during the extraction process. The interaction mechanism between Pluronic F68 and phycocyanin was systematically studied using FT-IR and fluorescence analysis. This was further complemented by static and dynamic calculation of molecular motion through molecular docking and molecular dynamics simulation, indicating that hydrophobic segment of Pluronic F68 played a key role in the binding process with phycocyanin.
本研究开发了一种从螺旋藻中富集和纯化藻蓝蛋白的可持续环保方法。该方法利用温度敏感聚合物泊洛沙姆 F68 在水相双溶剂体系中实现。评估了基于温度敏感聚合物的双相溶剂体系的相行为。通过单因素实验和响应面法优化了提取条件。在最佳条件下，上富聚合物相可回收用于可持续藻蓝蛋白提取，第 3 次提取循环的得率为 3.23。在提取过程中，泊洛沙姆 F68 可以有效地回收和再利用。通过傅里叶变换红外光谱和荧光分析系统研究了泊洛沙姆 F68 与藻蓝蛋白的相互作用机制。通过分子对接和分子动力学模拟对分子运动的静态和动态计算进一步补充，表明泊洛沙姆 F68 的疏水段在与藻蓝蛋白的结合过程中起关键作用。
