Divsion of Infectious Diseases and Global Public Health, Department of Medicine, University of California, San Diego, La Jolla, CA, USA.
Department of Microbiology and Molecular Biology, Chungnam National University, Yuseong-gu, Daejeon, Republic of Korea.
Methods Mol Biol. 2024;2837:59-66. doi: 10.1007/978-1-0716-4027-2_6.
Of all the chemical modifications of RNAs, the N6-methyladenosine (mA) modification is the most prevalent and well-characterized RNA modification that is functionally implicated in a wide range of biological processes. The mA modification occurs in hepatitis B virus (HBV) RNAs and this modification regulates the HBV life cycle in several ways. Thus, understanding the mechanisms underlying m6A modification of HBV RNAs is crucial in understanding HBV infectious process and associated pathogenesis. Here, we describe the currently utilized method in the detection and characterization of m6A-methylated RNAs during viral infection.
在所有 RNA 的化学修饰中，N6-甲基腺嘌呤（m6A）修饰是最普遍和特征最明显的 RNA 修饰，它在广泛的生物学过程中具有功能相关性。m6A 修饰发生在乙型肝炎病毒（HBV）RNA 中，这种修饰以多种方式调节 HBV 生命周期。因此，了解 HBV RNA 中 m6A 修饰的机制对于理解 HBV 感染过程和相关发病机制至关重要。在这里，我们描述了在病毒感染过程中检测和表征 m6A 甲基化 RNA 时目前使用的方法。
