Wayne L G
Crit Rev Microbiol. 1985;12(3):185-222. doi: 10.3109/10408418509104429.
Although techniques based on immunologic or chromatographic analyses have been described for identifying mycobacteria in clinical laboratories, most microbiologists continue to rely on a series of specialized physiological and biochemical tests for this purpose. The recognition of additional significant species over the past decade has required the addition of more tests to the battery used for mycobacterial identification. This paper will review briefly the taxonomic status of species likely to be encountered in clinical specimens and the most useful tests for characterizing them. Strategies will be presented for using these tests in the most efficient way to provide optimal resolution of taxa without use of an unreasonably large battery of tests. A brief survey of techniques that may become more practical in the future will also be included.
尽管临床实验室已描述了基于免疫分析或色谱分析的技术用于鉴定分枝杆菌,但大多数微生物学家仍继续依赖一系列专门的生理和生化试验来进行此项工作。在过去十年中,由于发现了更多重要的菌种,因此在用于分枝杆菌鉴定的试验组合中需要增加更多的试验。本文将简要回顾临床标本中可能遇到的菌种的分类地位以及用于鉴定它们的最有用的试验。还将介绍如何以最有效的方式使用这些试验,以便在不使用过多试验组合的情况下实现对分类单元的最佳分辨。本文还将简要介绍一些未来可能会更实用的技术。