Song Dongxu, Shi Youhui, Ji HengQing, Xia Yuxian, Peng Guoxiong
Genetic Engineering Research Center, School of Life Sciences, Chongqing University, Chongqing, China.
Chongqing Engineering Research Center for Fungal Insecticide, Chongqing, China.
Front Microbiol. 2019 Aug 21;10:1946. doi: 10.3389/fmicb.2019.01946. eCollection 2019.
As a CH type zinc finger transcription factor, is the key in Carbon Catabolism Repression (CCR) pathway, which negatively regulates the genes in carbon sources utilization. As conidiation in filamentous fungi is affected by nutritional conditions, may contribute to fungal conidiation, which has been well studied in filamentous fungi, especially spp., but researches on entomopathogenic fungi are not enough. In this study, we found a homologous gene in , and the deletion strain showed delayed conidiation, significant decrease in conidial yield, and 96.88% lower conidial production, when compared with the wild-type strain, and the normal conidiation and microcycle conidiation pattern shift was blocked. RT-qPCR showed that the transcription levels of the genes and (related to asexual development) were significantly altered, and those of most of the conidiation-related genes were higher in Δ strain. The results of RNA-Seq revealed that regulated the two conidiation patterns by mediating genes related to cell cycle, cell division, cell wall, and cell polarity. In conclusion, , as a core regulatory gene in conidiation, provides new insight into the mechanism of conidiation in entomopathogenic fungi.
作为一种CH型锌指转录因子，是碳代谢阻遏（CCR）途径中的关键因子，该途径对碳源利用相关基因起负调控作用。由于丝状真菌的分生孢子形成受营养条件影响，在丝状真菌中已有充分研究，特别是在某些种属中，但对昆虫病原真菌的研究还不够。在本研究中，我们在中发现了一个同源基因，与野生型菌株相比，缺失菌株的分生孢子形成延迟，分生孢子产量显著降低，产孢量降低了96.88%，正常的分生孢子形成和微循环分生孢子形成模式转变受阻。RT-qPCR显示，与无性发育相关的基因和的转录水平发生了显著变化，并且在Δ菌株中，大多数与分生孢子形成相关的基因转录水平更高。RNA-Seq结果表明，通过介导与细胞周期、细胞分裂、细胞壁和细胞极性相关的基因来调控两种分生孢子形成模式。总之，作为分生孢子形成中的核心调控基因，为昆虫病原真菌分生孢子形成机制提供了新的见解。
