School of Environmental and Chemical Engineering, Chongqing Three Gorges University, 404120, Chongqing, China.
School of Teacher Education, Chongqing Three Gorges University, 404120, Chongqing, China.
Dokl Biochem Biophys. 2024 Jun;516(1):58-65. doi: 10.1134/S1607672924600180. Epub 2024 May 9.
The objectives of this study were to investigate the anti-fatigue effects of Paris polyphylla polysaccharide component 1 (PPPm-1) and explore its mechanisms. A mouse model of exercise-induced fatigue was established by weight-bearing swimming to observe the effects of different concentrations of PPPm-1 on weight-bearing swimming time. The anti-fatigue effect of PPPm-1 was determined by the effects of contraction amplitude, contraction rate, and diastolic rate of the frog gastrocnemius muscle in vivo before and after infiltration with 5 mg/mL PPPm-1. The effects of PPPm-1 on the contents of blood lactate, serum urea nitrogen, hepatic glycogen, muscle glycogen in the exercise fatigue model of mice, and acetylcholine (ACh) content and acetylcholinesterase (AChE) activity at the junction of the frog sciatic nerve-gastrocnemius under normal physiological, and Na-K-ATPase and Ca-Mg-ATPase activities of the frog gastrocnemius were determined by enzyme-linked immunosorbent assay (ELISA), to investigate the anti-fatigue mechanisms of PPPm-1. The results showed that PPPm-1 could significantly prolong the weight-bearing swimming time in mice (P < 0.01), decrease the contents of blood lactate and serum urea nitrogen, increase the contents of the hepatic glycogen and muscle glycogen of mice after exercise fatigue compared with those of the control group, and there was extremely significant difference in most indicators (P < 0.01). The 5 mg/mL of PPPm-1 could significantly promote the contraction amplitude, contraction rate, and relaxation rate of the gastrocnemius muscle in the frogs, and the content of ACh at the junction of the frog sciatic nerve-gastrocnemius (P < 0.01), but it had obvious inhibitory effetc on the activity of AChE at the junction of the frog sciatic nerve-gastrocnemius (P < 0.01). PPPm-1 could increase the Na-K-ATPase and Ca-Mg-ATPase activities of gastrocnemius in the frogs (for Ca-Mg-ATPase, P < 0.01). The above results suggested that the PPPm-1 had a good anti-fatigue effect, and its main mechanisms were related to improving endurance and glycogen reserve, reducing glycogen consumption, lactate and serum urea nitrogen accumulation, and promoting Ca influx.
本研究的目的是探讨重楼多糖成分 1（PPPm-1）的抗疲劳作用，并探讨其机制。通过负重游泳建立运动性疲劳小鼠模型，观察不同浓度 PPPm-1 对负重游泳时间的影响。通过 5mg/mL PPPm-1 浸润前后体内青蛙腓肠肌收缩幅度、收缩率和舒张率的变化，确定 PPPm-1 的抗疲劳作用。通过酶联免疫吸附试验（ELISA）测定 PPPm-1 对运动性疲劳模型小鼠血乳酸、血清尿素氮、肝糖原、肌糖原含量以及正常生理状态下青蛙坐骨神经-腓肠肌接头处乙酰胆碱（ACh）含量和乙酰胆碱酯酶（AChE）活性的影响，以及青蛙腓肠肌 Na-K-ATPase 和 Ca-Mg-ATPase 活性，探讨 PPPm-1 的抗疲劳机制。结果表明，PPPm-1 能显著延长小鼠负重游泳时间（P<0.01），降低运动性疲劳后小鼠血乳酸和血清尿素氮含量，增加肝糖原和肌糖原含量，多数指标差异极显著（P<0.01）。5mg/mL 的 PPPm-1 能显著促进青蛙腓肠肌的收缩幅度、收缩率和舒张率，显著增加青蛙坐骨神经-腓肠肌接头处 ACh 的含量（P<0.01），但对 AChE 的活性有明显的抑制作用（P<0.01）。PPPm-1 能提高青蛙腓肠肌的 Na-K-ATPase 和 Ca-Mg-ATPase 活性（对于 Ca-Mg-ATPase，P<0.01）。上述结果表明，PPPm-1 具有良好的抗疲劳作用，其主要机制与提高耐力和糖原储备、减少糖原消耗、乳酸和血清尿素氮积累以及促进 Ca 内流有关。
