Pérez-Anzúrez Gustavo, Mendoza-de Gives Pedro, Alonso-Díaz Miguel Ángel, von Son-de Fernex Elke, Paz-Silva Adolfo, López-Arellano María Eugenia, Olmedo-Juárez Agustín
Laboratory of Helminthology, National Centre for Disciplinary Research in Animal Health and Innocuity (CENID-SAI), National Institute for Research in Forestry, Agriculture and Livestock, INIFAP-SADER, Jiutepec 62550, Mexico.
Production Sciences and Animal Health, Faculty of Veterinary Medicine and Zootechnics, National Autonomous University of Mexico, Coyoacán 04510, Mexico.
Pathogens. 2024 Jul 16;13(7):588. doi: 10.3390/pathogens13070588.
Nematophagous fungi (NF) form part of the soil microbiota and are natural enemies of nematodes, helping to regulate nematode populations. A verticillate NF isolated from soil from Tepalcingo, Mexico, was morphologically and molecularly characterised. This fungus was cultured in two different liquid media-Czapek-Dox broth (CzDoxB) and sweet potato dextrose broth (SPDB)-for 21 days. The ovicidal (OA) and larvicidal (LA) activities of fungal liquid culture filtrates (LCFs) were assessed in 96-well microtitre plates at different concentrations against after 48 h. The morphological and molecular identification revealed the presence of . Additionally, the groups of compounds associated with nematocidal activity were determined from a qualitative chemical profile (QCP) using different reagents. The highest OA of the LCFs was obtained at 25 mg/mL from SPDB and CzDoxB and amounted to 97.2 and 99.06%, respectively. Meanwhile, the highest LA recorded with these LCFs at 100 mg/mL was 54.27% and 96.8%, respectively. The QCP revealed the presence of alkaloids and tannins in both LCFs that have previously been associated with nematocidal activity. exerted an important effect on . and could be of significance in future studies focused on the control and prevention of haemonchosis in small ruminants.
捕食线虫真菌（NF）是土壤微生物群的一部分，是线虫的天敌，有助于调节线虫种群数量。从墨西哥特帕尔辛戈的土壤中分离出一种轮枝状捕食线虫真菌，并对其进行了形态学和分子特征分析。将这种真菌在两种不同的液体培养基——察氏肉汤（CzDoxB）和甘薯葡萄糖肉汤（SPDB）中培养21天。在96孔微量滴定板中，于48小时后评估不同浓度的真菌液体培养滤液（LCF）对[此处原文缺失具体受试对象]的杀卵（OA）和杀幼虫（LA）活性。形态学和分子鉴定表明存在[此处原文缺失具体内容]。此外，使用不同试剂从定性化学图谱（QCP）中确定与杀线虫活性相关的化合物组。来自SPDB和CzDoxB的LCF在25 mg/mL时获得最高OA，分别为97.2%和99.06%。同时，这些LCF在100 mg/mL时记录的最高LA分别为54.27%和96.8%。QCP显示两种LCF中均存在生物碱和单宁，此前已发现它们与杀线虫活性有关。[此处原文缺失具体内容]对[此处原文缺失具体内容]产生了重要影响。[此处原文缺失具体内容]，并且在未来专注于小型反刍动物血矛线虫病防治的研究中可能具有重要意义。
