墨西哥首次发现（麦角菌科，肉座菌目）：形态学和分子特征，其液体培养滤液对的杀线虫活性及蛋白酶活性

First Record of (Cordycipitaceae, Hypocreales) in Mexico: Morphological and Molecular Characterisation, Nematocidal Activity of Its Liquid Culture Filtrates against and Protease Activity.

作者信息

Pérez-Anzúrez Gustavo, Mendoza-de Gives Pedro, Olmedo-Juárez Agustín, López-Arellano María Eugenia, Bautista-García Génesis Andrea, Ocampo-Gutiérrez Ana Yuridia, von Son-de Fernex Elke, Alonso-Díaz Miguel Ángel, Delgado-Núñez Edgar Jesús, Paz-Silva Adolfo

机构信息

Laboratory of Helminthology, National Centre for Disciplinary Research in Animal Health and Innocuity (CENID-SAI), National Institute for Research in Forestry, Agriculture and Livestock-Ministry of Agriculture and Rural Development, (INIFAP-SADER), Jiutepec 62550, Mexico.

Production Sciences and Animal Health, Faculty of Veterinary Medicine and Zootechnics, National Autonomous University of Mexico, Coyoacán, Ciudad de México 04510, Mexico.

出版信息

J Fungi (Basel). 2024 Jan 9;10(1):56. doi: 10.3390/jof10010056.

DOI:10.3390/jof10010056

PMID:38248965

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10817658/

Abstract

This is the first record of the fungus in Mexico. The isolate was taxonomically characterised and cultured in potato dextrose broth (PDB), Czapek-Dox broth (CzDoxB), and sweet potato dextrose broth (SPDB) to obtain its filtrates (FLCF). The nematocidal activity (NA) of three FLCF concentrations was assessed against L3. Protease activity (PA) was assessed with SDS-PAGE, followed by a zymogram. The NA of the FLCF reached 94.43% in PDB and 95.82% in CzDoxB, respectively, at 100 mg/mL. Lower mortality (64%) was found in SPDB at 100 mg/mL. SDS-PAGE showed bands (in PBS) of ~25, ~40, and ~55 kDa. The zymogram showed protein bands (PBs) with PA in the media, including PBs of ~14, ~40, and ~55 kDa. This study establishes the basis for exploring the potential use of this fungus against . , which is considered the most pathogenic parasite affecting lambs.

摘要

这是该真菌在墨西哥的首次记录。对分离株进行了分类学鉴定，并在马铃薯葡萄糖肉汤（PDB）、察氏肉汤（CzDoxB）和甘薯葡萄糖肉汤（SPDB）中进行培养以获得其滤液（FLCF）。评估了三种FLCF浓度对L3的杀线虫活性（NA）。用SDS-PAGE评估蛋白酶活性（PA），随后进行酶谱分析。在100 mg/mL时，PDB中FLCF的NA分别达到94.43%，CzDoxB中达到95.82%。在100 mg/mL时，SPDB中的死亡率较低（64%）。SDS-PAGE显示（在PBS中）约25、约40和约55 kDa的条带。酶谱分析显示培养基中具有PA的蛋白条带（PBs），包括约14、约40和约55 kDa的PBs。本研究为探索该真菌对[此处原文缺失相关内容]的潜在用途奠定了基础，[此处原文缺失相关内容]被认为是影响羔羊的最具致病性的寄生虫。