墨西哥离蠕孢虫（Orbiliales）的节丛孢属（Arthrobotrys）菌株的研究：液体培养滤液对捻转血矛线虫（Trichostrongylidae）的捕食行为和杀线虫活性、蛋白质图谱和真菌成分。

Study of a Mexican isolate of Arthrobotrys musiformis (Orbiliales): Predatory behavior and nematocidal activity of liquid culture filtrates against Haemonchus contortus (Trichostrongylidae), protein profile and myco-constituent groups.

机构信息

Laboratory of Helminthology, National Centre for Disciplinary Research in Animal Health and Innocuity (CENID-SAI), National Institute for Research in Forestry, Agriculture and Livestock, INIFAP-SADER, Morelos, Jiutepec CP 62550, Mexico; Department of Research, Polytechnic University of Morelos State (UPEMOR), Boulevard Paseo Cuauhnáhuac No. 566, Colonia Lomas del Texcal, 62550 Jiutepec, Morelos, Mexico.

出版信息

Fungal Biol. 2023 Oct-Nov;127(10-11):1345-1361. doi: 10.1016/j.funbio.2023.09.001. Epub 2023 Sep 29.

DOI:10.1016/j.funbio.2023.09.001

PMID:37993246

Abstract

A Mexican isolate of the nematophagous fungus Arthrobotrys musiformis was obtained from a soil sample from the Chapultepec ecological reserve zone, in Cuernavaca, Morelos, Mexico. This isolate demonstrated an important predatory activity (74.9%) against the parasitic nematode Haemonchus contortus (L3) and its fungal liquid culture filtrates (LCF) grown in two media showed the following highest nematocidal activities (NA): In Czapek-DoxBroth (CzDoxB) 80.66% and potato-dextrose broth (PDB) 49.84%. Additionally, two major compounds derived from carboxylic acids and two derivates from alkane group were identified by GC-MS. These compounds have been associated to many biological activities. On the other hand, the protein profile analysis by SDS-electrophoresis followed by a zymogram revealed a 10 kDa protein with protease activity. This study provides important information for future experiments focused to explore the potential use of this protein as well as the identified bioactive compounds presents in the LCF as potential candidates against sheep haemonchosis.

摘要

从墨西哥莫雷洛斯州库埃纳瓦卡查普尔特佩克生态保护区的土壤样本中获得了一种捕食性真菌节丛孢菌（Arthrobotrys musiformis）的墨西哥分离株。该分离株对寄生性线虫捻转血矛线虫（L3）表现出重要的捕食活性（74.9%），其在两种培养基中生长的真菌液体培养滤液（LCF）表现出以下最高的杀线虫活性（NA）：在察氏培养基（CzDoxB）中为 80.66%，在土豆葡萄糖肉汤（PDB）中为 49.84%。此外，通过 GC-MS 鉴定了两种来源于羧酸的主要化合物和两种来源于烷烃的衍生物。这些化合物与许多生物活性有关。另一方面，通过 SDS-电泳和同工酶分析的蛋白质图谱分析显示，一种具有蛋白酶活性的 10 kDa 蛋白质。这项研究为未来的实验提供了重要信息，这些实验旨在探索将这种蛋白质以及 LCF 中存在的鉴定出的生物活性化合物作为抗绵羊血矛线虫病的潜在候选物的潜在用途。