Coffey Emily L, Becker Zoe W, Gomez Andres M, Ericsson Aaron C, Churchill Julie A, Burton Erin N, Granick Jennifer L, Lulich Jody P, Furrow Eva
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN 55108, USA.
Department of Animal Science, College of Food, Agricultural, and Natural Resource Sciences, University of Minnesota, Saint Paul, MN 55108, USA.
Vet Sci. 2024 Jun 27;11(7):286. doi: 10.3390/vetsci11070286.
Nutrition plays an important role in shaping the gut microbiome composition, although the impact of diet on the urinary microbiome (i.e., urobiome) remains unknown. The aim of this pilot study was to discover how nutritional features affect the diversity and composition of the urobiome in dogs. Dietary histories were obtained for 15 clinically healthy adult dogs, including limited nutrient (protein, fat, crude fiber), commercial diet brand, and dietary diversity profiles. The urine samples were collected via cystocentesis, followed by sequencing of the bacterial 16S rRNA gene. The data were analyzed to determine associations between major nutrients and dietary sources with the urobiome's composition. The protein, fat, and crude fiber contents had no statistically significant effect on the alpha or beta diversity. However, the beta diversity values differed (PERMANOVA; = 0.017, R = 0.10) between dogs fed one commercial diet brand compared to dogs consuming any other brand. The beta diversity values also differed ( = 0.019, R = 0.10) between dogs consuming more diverse daily diets compared to those consuming less diverse diets (≥3 or <3 unique food sources, respectively). Overall, the results of this pilot study suggest that diet might impact the urobiome and support further exploration of the relationship between diet and the urobiome's composition in dogs.
营养在塑造肠道微生物群组成方面起着重要作用，尽管饮食对泌尿微生物群（即泌尿生物群）的影响尚不清楚。这项初步研究的目的是发现营养特征如何影响犬类泌尿生物群的多样性和组成。获取了15只临床健康成年犬的饮食史，包括有限营养素（蛋白质、脂肪、粗纤维）、商业饮食品牌和饮食多样性概况。通过膀胱穿刺术收集尿液样本，随后对细菌16S rRNA基因进行测序。分析数据以确定主要营养素和饮食来源与泌尿生物群组成之间的关联。蛋白质、脂肪和粗纤维含量对α或β多样性没有统计学上的显著影响。然而，与食用其他任何品牌的犬相比，食用一种商业饮食品牌的犬之间的β多样性值有所不同（PERMANOVA；P = 0.017，R = 0.10）。与饮食多样性较少（分别为≥3种或<3种独特食物来源）的犬相比，日常饮食更多样化的犬之间的β多样性值也有所不同（P = 0.019，R = 0.10）。总体而言，这项初步研究的结果表明，饮食可能会影响泌尿生物群，并支持进一步探索犬类饮食与泌尿生物群组成之间的关系。
