Shandong Institute of Medical Device and Pharmaceutical Packaging Inspection, Jinan, Shandong, China.
NMPA Key Laboratory for Safety Evaluation of Biomaterials and Medical Devices, Jinan, Shandong, China.
Microbiol Spectr. 2024 Sep 3;12(9):e0023724. doi: 10.1128/spectrum.00237-24. Epub 2024 Jul 26.
Central venous catheters (CVCs) are widely used for intravenous medication administration. However, biofilm formation along the catheter surface is the main most important cause of catheter-related bloodstream infections. Nowadays, several antimicrobial-coated catheters are available to prevent biofilm development. In this study, we introduced a new dynamic model to evaluate the antimicrobial activity against bacterial biofilms on CVCs. Rifampicin-minocycline-coated catheters and control catheters without antimicrobial component were assembled into the model to test the antimicrobial activity on external surface and internal surface. After 1 h irrigation of or preculture and 23 h irrigation of Trypticase Soy Broth, the viable adherent organism was collected and counted. The enumeration results showed that the number of bacteria attached to antibacterial catheter was significantly less than that of the control catheter, both on external surface ( < 0.05) and internal surface ( < 0.05). The results were further confirmed by the scanning electron microscopy. In conclusion, the dynamic model can be applied to evaluate the antimicrobial activity against bacterial biofilms grown on the external and internal surfaces of CVCs used in clinical practice.IMPORTANCEFor the first time, a new dynamic model was constructed to evaluate the antimicrobial activity against bacterial biofilms on central venous catheters (CVCs) on both external surface and internal surface. This model could be applied to evaluate the antimicrobial activity against bacterial biofilms not only on CVCs but also other types of catheters.
中心静脉导管（CVC）广泛用于静脉内药物给药。然而，沿导管表面形成生物膜是导管相关性血流感染的主要原因。如今，有几种抗菌涂层导管可用于防止生物膜的发展。在这项研究中，我们引入了一种新的动态模型来评估对 CVC 上细菌生物膜的抗菌活性。利福平-米诺环素涂层导管和没有抗菌成分的对照导管被组装到模型中，以测试对外表面和内表面的抗菌活性。在 1 h 冲洗 或预培养物和 23 h 冲洗胰蛋白酶大豆肉汤后，收集并计数可存活的附着生物。计数结果表明，附着在抗菌导管上的细菌数量明显少于对照导管，无论是在外表面（<0.05）还是内表面（<0.05）。扫描电子显微镜进一步证实了这一结果。总之，该动态模型可用于评估临床实践中使用的 CVC 外部和内部表面上生长的细菌生物膜的抗菌活性。
重要性：
这是首次构建了一种新的动态模型来评估对 CVC 上细菌生物膜的抗菌活性，包括外表面和内表面。该模型不仅可用于评估 CVC 上的细菌生物膜的抗菌活性，还可用于评估其他类型导管上的抗菌活性。
