河口黄杆菌新种，一种从珠江口分离出的新物种。

Flavobacterium aestuarii sp. nov., a novel species isolated from the Pearl River Estuary.

作者信息

Li Qi, Peng Zi-Qi, Wang Hong-Chuan, Dong Lei, Guo Dan-Yuan, Wang Pan-Deng, Luo Xiao-Qing, Luo Zhen-Hao, Yang Zi-Wen, Li Wen-Jun

机构信息

School of Life Sciences, Conservation and Utilization Laboratory of Mountain Characteristic Resources in Guangdong Province, Jiaying University, Meizhou, 514015, People's Republic of China.

State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Stress Biology and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat‑Sen University, Guangzhou, 510275, People's Republic of China.

出版信息

Antonie Van Leeuwenhoek. 2025 Jul 2;118(8):101. doi: 10.1007/s10482-025-02109-5.

DOI:10.1007/s10482-025-02109-5

PMID:40601161

Abstract

Strain SYSU M80003, a Gram-stain-negative and aerobic bacterium, was isolated from water sample of the Pearl River Estuary, Guangzhou, Guangdong, PR China. The strain could grow at 4-37 °C (optimal: 28 °C), pH 6.0-8.0 (optimal: 7.0), and with 0-1% NaCl (w/v, optimal: 0%). The main fatty acids were anteiso-C, Summed Feature 3 (C ω7c and/or C ω6c) and iso-C. The predominant respiratory quinone was MK-6. The polar lipids contained phosphatidylethanolamine, three unidentified aminolipids, two unidentified aminophospholipids, and two unidentified polar lipids. The genomic DNA G + C content was 35.06%. Phylogenetic analysis based on the 16S rRNA gene revealed that strain SYSU M80003 was closely related to Flavobacterium aquicola JCM 30987 with similarity of 97.28%. Furthermore, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain SYSU M80003 and F. aquicola JCM 30987 were 83.8% and 28.1%, respectively. Based upon the consensus of phylogenetic and ANI analyses, we conclude that strain SYSU M80003 represents a novel species within the genus Flavobacterium, for which the name Flavobacterium aestuarii sp. nov. is proposed. The type strain is SYSU M80003 (= GDMCC 1.4521 = KCTC 102267).

摘要

菌株SYSU M80003是一种革兰氏阴性需氧细菌，从中国广东省广州市珠江口的水样中分离得到。该菌株能在4-37°C（最适温度：28°C）、pH 6.0-8.0（最适pH：7.0）以及含0-1% NaCl（w/v，最适浓度：0%）的条件下生长。主要脂肪酸为anteiso-C、特征性总和3（C ω7c和/或C ω6c）以及iso-C。主要呼吸醌为MK-6。极性脂质包含磷脂酰乙醇胺、三种未鉴定的氨基脂质、两种未鉴定的氨基磷脂以及两种未鉴定的极性脂质。基因组DNA的G+C含量为35.06%。基于16S rRNA基因的系统发育分析表明，菌株SYSU M80003与水生黄杆菌JCM 30987密切相关，相似度为97.28%。此外，菌株SYSU M80003与水生黄杆菌JCM 30987之间的平均核苷酸同一性（ANI）和数字DNA-DNA杂交（dDDH）值分别为83.8%和28.1%。基于系统发育分析和ANI分析的结果，我们得出结论，菌株SYSU M80003代表黄杆菌属内的一个新物种，为此提出新名称河口黄杆菌（Flavobacterium aestuarii sp. nov.）。模式菌株为SYSU M80003（=GDMCC 1.4521 = KCTC 102267）。