Department of Pathology, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.
Department of Pathology, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea;
Anticancer Res. 2024 Aug;44(8):3381-3395. doi: 10.21873/anticanres.17159.
BACKGROUND/AIM: Studies on the differences in gene expression characteristics according to lymph node metastasis in breast carcinoma are lacking. This study aimed to compare the spatially resolved transcriptomic profiles between node-positive and negative breast carcinomas.
Eight patients with breast carcinoma were included, and digital spatial profiling and bioinformatic analysis were conducted to investigate the spatial transcriptomes.
In the epithelial compartment, the top two most up-regulated genes were nuclear receptor subfamily 4 group A member 1 (NR4A1) and Jun proto-oncogene, activating protein-1 transcription factor subunit (JUN). The gene ontology (GO) enrichment analysis of the node-positive group revealed a significant up-regulation of genes associated with myeloid differentiation, mononuclear cell differentiation, and hematopoietic regulation. The gene set enrichment analysis (GSEA) revealed significant enrichment of gene sets associated with the regulation of inflammatory cytokines in both the epithelial and stromal compartments of the node-positive group.
Our study highlights significant differences in gene expression profiles and spatially resolved transcriptional activities between node-positive and negative breast carcinomas. These findings underscore the importance of developing personalized treatment strategies for lymph node metastasis of breast carcinoma.
背景/目的：关于乳腺癌淋巴结转移的基因表达特征差异的研究较少。本研究旨在比较淋巴结阳性和阴性乳腺癌之间的空间转录组图谱。
纳入 8 例乳腺癌患者，进行数字空间分析和生物信息学分析以研究空间转录组。
在上皮细胞区室中，上调最明显的前两个基因是核受体亚家族 4 组 A 成员 1（NR4A1）和 Jun 原癌基因，激活蛋白 1 转录因子亚基（JUN）。淋巴结阳性组的基因本体（GO）富集分析显示，与髓样分化、单核细胞分化和造血调节相关的基因显著上调。基因集富集分析（GSEA）显示，在淋巴结阳性组的上皮和基质区室中，与炎症细胞因子调节相关的基因集均显著富集。
本研究强调了淋巴结阳性和阴性乳腺癌之间基因表达谱和空间转录活性的显著差异。这些发现强调了为乳腺癌淋巴结转移制定个体化治疗策略的重要性。
