Differential Impact of Valproic Acid on , , , , and Expression in Adult Glioblastoma Cells.

Valproic acid (VPA) has anticancer, anti-inflammatory, and epigenetic effects. The study aimed to determine the expression of carcinogenesis-related , , , and in adult glioblastoma U87 MG and T98G cells and the effects of 0.5 mM, 0.75 mM, and 1.5 mM doses of VPA. RNA gene expression was determined by RT-PCR. was used as a control. U87 and T98G control cells do not express or was expressed in U87 control but not in T98G control cells. The expression in the U87 control was significantly lower than in the T98G control. T98G control cells showed significantly higher expression than U87 control cells. VPA treatment did not affect expression in U87 cells, whereas treatment dose-dependently increased expression in T98G cells. Treatment with 1.5 mM VPA induced expression in U87 cells, while treatment of T98G cells with VPA did not affect expression. Treatment of U87 cells with VPA significantly increased expression. VPA increases expression depending on the VPA dose. expression was significantly increased only in the U87 1.5 mM VPA group. Tested VPA doses significantly increased expression in T98G cells. When approaching treatment tactics, assessing the cell's sensitivity to the agent is essential.