Zhu Han, Ye Zhenli, Xu Zhengru, Wei Li
Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou 571158, China.
Hainan Observation and Research Station of Dongzhaigang Mangrove Wetland Ecosystem, Haikou 571129, China.
Life (Basel). 2024 Jun 24;14(7):797. doi: 10.3390/life14070797.
The eukaryotic microalga represents a promising bioresource for the production of biofuels and pharmaceuticals. Urea, a crucial nutrient for the photosynthetic , stimulates the accumulation of substances such as lipids, which influence growth and physiology. However, the specific mechanisms by which responds and adapts to urea addition remain unknown. High-throughput mRNA sequencing and differential gene expression analysis under control and urea-added conditions revealed significant metabolic changes. This involved the differential expression of 2104 genes, with 1354 being upregulated and 750 downregulated, resulting in the reprogramming of crucial pathways such as carbon and nitrogen metabolism, photosynthesis, and lipid metabolism. The results specifically showed that genes associated with photosynthesis in were significantly downregulated, particularly those related to light-harvesting proteins. Interestingly, urea absorption and transport may depend not only on specialized transport channels such as urease but also on alternative transport channels such as the ABC transporter family and the CLC protein family. In addition, urea caused specific changes in carbon and lipid metabolism. Genes associated with the Calvin cycle and carbon concentration mechanisms were significantly upregulated. In lipid metabolism, the expression of genes associated with lipases and polyunsaturated fatty acid synthesis was highly activated. Furthermore, the expression of several genes involved in the tricarboxylic acid cycle and folate metabolism was enhanced, making important contributions to energy supply and the synthesis and modification of genes and macromolecules. Our observations indicate that actively and dynamically regulates the redistribution of carbon and nitrogen after urea addition, providing references for further research on the effects of urea on .
真核微藻是生产生物燃料和药物的一种有前景的生物资源。尿素是光合作用的关键养分，能刺激脂质等物质的积累，而这些物质会影响生长和生理机能。然而，其对添加尿素做出反应和适应的具体机制仍不清楚。在对照和添加尿素条件下进行的高通量mRNA测序和差异基因表达分析揭示了显著的代谢变化。这涉及2104个基因的差异表达，其中1354个上调，750个下调，导致碳和氮代谢、光合作用及脂质代谢等关键途径的重新编程。结果具体表明，该微藻中与光合作用相关的基因显著下调，尤其是那些与光捕获蛋白相关的基因。有趣的是，尿素的吸收和运输可能不仅取决于诸如脲酶等专门的运输通道，还取决于诸如ABC转运蛋白家族和CLC蛋白家族等其他运输通道。此外，尿素引起了碳和脂质代谢的特定变化。与卡尔文循环和碳浓缩机制相关的基因显著上调。在脂质代谢中，与脂肪酶和多不饱和脂肪酸合成相关的基因表达被高度激活。此外，参与三羧酸循环和叶酸代谢的几个基因的表达增强，为能量供应以及基因和大分子的合成与修饰做出了重要贡献。我们的观察结果表明，该微藻在添加尿素后积极且动态地调节碳和氮的重新分配，为进一步研究尿素对该微藻的影响提供了参考。
