Ramadan Intermittent Fasting and Plasma Volume Variations in Individuals with Different Body Weights.

: There is increasing awareness of the physiological effects of Ramadan intermittent fasting (RIF) in obese subjects. However, there are no data on the effects of RIF on plasma volume changes (ΔPV) in individuals with different body weights. : This study investigated the effects of RIF on ΔPV in normal-weight (NW) and overweight (OW) adult men, and adult men with obesity (OB) and severe obesity (SO). : Thirty-two male subjects (32) were divided into four groups ( = 8 per group) according to their body mass index (BMI): normal weight (NW) (BMI < 25 kg/m; age = 27.4 ± 3.8), overweight (OW) (BMI between 25 and 29.9 kg/m; age = 26.8 ± 3.7), obese subjects (OB) (BMI between 30 and 34.9 kg/m; age = 25.6 ± 2.9), and severely obesity (SO) (BMI between 35 and 40 kg/m; age = 24.0 ± 4.1). Blood samples were collected for 24 h on 4 different occasions, at T0 before the start of the Ramadan month, at T1 15 days after the start of Ramadan, at T2 one day after the end of Ramadan, and at T3 on the 21st day after the end of Ramadan to determine ΔPV. All groups completed their fasting rituals for the 30 days of Ramadan. : A significant group × time effect occurred for body mass ( = 0.001; ES = 0.53), BMI ( = 0.001; ES = 0.53), and body fat percentage ( = 0.001; ES = 0.52). Post hoc tests indicated reductions in body mass in OB and SO at T1 ( = 0.03; ES = 0.21 and = 0.002; ES = 0.12) and T2 ( = 0.03; ES = 0.31 and = 0.02; ES = 0.23), reductions in BMI in OB and SO at T1 ( = 0.04; ES = 0.35 and = 0.03; ES = 0.42) and T2 ( = 0.03; ES = 0.52 and = 0.005; ES = 0.48), and reductions in body fat percentage only in OB AT T1 ( = 0.002; ES = 0.31) and T2 ( = 0.001; ES = 0.17). A significant group × time effect occurred for hematocrit ( = 0.02; ES = 0.34), hemoglobin ( = 0.01; ES = 0.35), and ΔPV ( = 0.02; ES = 0.18). Post hoc tests indicated increases in hematocrit in OB at T2 ( = 0.03; ES = 0.36) and hemoglobin in OB and SO at T1 ( = 0.03; ES = 0.35 and = 0.002; ES = 0.32) and T2 ( = 0.003; ES = 0.21 and = 0.002; ES = 0.33). There were also increases in ΔPV in OB at T1 and T2 ( = 0.002; ES = 0.25 and = 0.003; ES = 0.22) and in SO only at T2 ( = 0.02; ES = 0.37). Contrast analysis indicated that NW was significantly lower than the grand mean of OW, Ob, and SO for all anthropometric and PVV variables (all < 0.05). : The effects of RIF on ΔPV and anthropometric characters was greater in obese individuals compared to normal-weight and overweight participants, suggesting that the improvements in body composition and ΔPV produced by RIF could positively influence obesity.