Vongnhay Vanessa, Shukla Mukund R, Ayyanath Murali-Mohan, Sriskantharajah Karthika, Saxena Praveen K
Department of Plant Agriculture, Gosling Research Institute for Plant Preservation, University of Guelph, Guelph, ON N1G 2W1, Canada.
Plants (Basel). 2024 Jul 22;13(14):2002. doi: 10.3390/plants13142002.
Plant growth regulators (PGRs) play a vital role in the induction of morphogenesis in vitro. Synthetic PGRs are commonly used to induce organogenesis and somatic embryogenesis from various explants, while natural substances are rarely utilized. This study aimed to enhance the regenerative response in leaf explants using Tulsi () leaf extract and to elucidate the biochemical interactions during modulation of endogenous plant growth regulators, including indole-3-acetic acid (IAA), abscisic acid (ABA), zeatin, and 6-(γ, γ-dimethylallylamino) purine (2iP). Tulsi leaf extract significantly improved shoot production through interactions between endogenous hormones and those present in the extract, which enhanced stress mitigation. The 20% Tulsi leaf extract treatment produced significantly more shoots than the control, coinciding with increased endogenous IAA and zeatin levels starting on day 10 in culture. Furthermore, ABA and zeatin concentrations increased on days 15 and 25, respectively, in the 20% Tulsi extract treatment, suggesting their role in the induction of somatic embryo-like structures. ABA likely acts as an activator of stress responses, encouraging the development of these structures. Additionally, 2iP was involved in the induction of both forms of regeneration in the 10% and 20% extract treatments, especially in combination with ABA. These results suggest that Tulsi leaf extract holds promising potential as a natural supplement for increasing plant regeneration in vitro and advancing our understanding of how natural extracts of plant origin can be harnessed to optimize plant regeneration processes in vitro.
植物生长调节剂（PGRs）在体外形态发生诱导中起着至关重要的作用。合成植物生长调节剂通常用于从各种外植体诱导器官发生和体细胞胚胎发生，而天然物质很少被利用。本研究旨在利用罗勒（）叶提取物增强叶片外植体的再生反应，并阐明在调节包括吲哚 - 3 - 乙酸（IAA）、脱落酸（ABA）、玉米素和6 - （γ，γ - 二甲基烯丙基氨基）嘌呤（2iP）在内的内源性植物生长调节剂过程中的生化相互作用。罗勒叶提取物通过内源性激素与提取物中存在的激素之间的相互作用显著提高了芽的产生，这增强了应激缓解。20%罗勒叶提取物处理产生的芽明显多于对照，这与培养第10天开始内源性IAA和玉米素水平的增加相吻合。此外，在20%罗勒提取物处理中，ABA和玉米素浓度分别在第15天和第25天增加，表明它们在诱导体细胞胚样结构中的作用。ABA可能作为应激反应的激活剂，促进这些结构的发育。此外，在10%和20%提取物处理中，2iP参与了两种再生形式的诱导，特别是与ABA结合时。这些结果表明，罗勒叶提取物作为一种天然补充剂，在提高植物体外再生以及推进我们对如何利用植物来源的天然提取物优化植物体外再生过程的理解方面具有广阔的潜力。
