Avilez-Montalvo Johny R, Quintana-Escobar Ana O, Méndez-Hernández Hugo A, Aguilar-Hernández Víctor, Brito-Argáez Ligia, Galaz-Ávalos Rosa M, Uc-Chuc Miguel A, Loyola-Vargas Víctor M
Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Calle 43, No. 130 × 32 y 34, Mérida 97205, Mexico.
Catedrático CONACYT, Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Mérida 97205, Mexico.
Plants (Basel). 2022 Aug 2;11(15):2013. doi: 10.3390/plants11152013.
Cytokinins (CK) are plant growth regulators involved in multiple physiological processes in plants. One less studied aspect is CK homeostasis (HM). The primary genes related to HM are involved in biosynthesis (IPT), degradation (CKX), and signaling (ARR). This paper demonstrates the effect of auxin (Aux) and CK and their cross talk in a embryogenic system. The transcriptome and RT-qPCR suggest that Aux in pre-treatment represses biosynthesis, degradation, and signal CK genes. However, in the induction, there is an increase of genes implicated in the CK perception/signal, indicating perhaps, as in other species, Aux is repressing CK, and CK are inducing per se genes involved in its HM. This is reflected in the endogenous concentration of CK; pharmacology experiments helped study the effect of each plant growth regulator in our SE system. We conclude that the Aux-CK balance is crucial to directing somatic embryogenesis in .
细胞分裂素（CK）是参与植物多种生理过程的植物生长调节剂。一个研究较少的方面是CK稳态（HM）。与HM相关的主要基因参与生物合成（IPT）、降解（CKX）和信号传导（ARR）。本文展示了生长素（Aux）和CK及其相互作用在一个胚胎发生系统中的作用。转录组和RT-qPCR表明，预处理中的Aux抑制生物合成、降解和信号CK基因。然而，在诱导过程中，参与CK感知/信号的基因增加，这可能表明，与其他物种一样，Aux抑制CK，而CK诱导参与其HM的自身基因。这反映在CK的内源浓度上；药理学实验有助于研究每种植物生长调节剂在我们的体细胞胚胎发生（SE）系统中的作用。我们得出结论，Aux-CK平衡对于指导[具体植物名称未给出]的体细胞胚胎发生至关重要。
