EZH2 及其调控长非编码 RNA 作为阿尔茨海默病血清生物标志物的作用。
The role of EZH2 and its regulatory lncRNAs as a serum-based biomarker in Alzheimer's disease.
机构信息
Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
Neurology Department, School of Medicine, Poursina Hospital, Guilan University of Medical Sciences, Rasht, Iran.
出版信息
Mol Biol Rep. 2024 Jul 29;51(1):866. doi: 10.1007/s11033-024-09802-0.
BACKGROUND
Long noncoding RNAs (lncRNAs) have become a hot topic in the human nervous system. Moreover, circulating lncRNAs have been suggested as possible biomarkers for central nervous system processes and neurodegenerative diseases. The present research aimed to highlight the role of plasma lncRNAs TUG1, FEZF1-AS1, and EZH2 gene as diagnostic biomarkers in Alzheimer's disease (AD).
METHODS
Plasma samples for the study were provided by 100 AD patients and 100 matched controls. Real-time quantitative reverse transcriptase PCR was used to determine the plasma level of the aforementioned lncRNAs. Furthermore, the plasma level of EZH2 protein in the participants' blood was determined using the ELISA technique.
RESULTS
In contrast to controls, down-regulation of the EZH2 gene and protein was reported in the plasma of patients with AD. Additionally, plasma samples from AD patients showed up-and-down-regulation of the lncRNAs TUG1 and FEZF1-AS1, respectively.
CONCLUSION
Our new findings suggest that the EZH2 gene, plasma lncRNA TUG1, and FEZF1-AS1 may contribute, as valuable biomarkers, to AD diagnosis.
背景
长链非编码 RNA（lncRNAs）已成为人类神经系统的热门话题。此外，循环 lncRNAs 被认为是中枢神经系统过程和神经退行性疾病的潜在生物标志物。本研究旨在强调血浆 lncRNAs TUG1、FEZF1-AS1 和 EZH2 基因作为阿尔茨海默病（AD）诊断生物标志物的作用。
方法
本研究提供了 100 例 AD 患者和 100 例匹配对照的血浆样本。采用实时定量逆转录 PCR 测定上述 lncRNAs 的血浆水平。此外，采用 ELISA 技术测定参与者血液中 EZH2 蛋白的血浆水平。
结果
与对照组相比，AD 患者的血浆中 EZH2 基因和蛋白表达下调。此外，AD 患者的血浆样本中 lncRNA TUG1 和 FEZF1-AS1 的表达分别上调和下调。
结论
我们的新发现表明，EZH2 基因、血浆 lncRNA TUG1 和 FEZF1-AS1 可能作为有价值的生物标志物，有助于 AD 的诊断。
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