Li Yangjie, Cavet Guy, Zare Richard N, Driver Taran
Department of Chemistry, Stanford University, Stanford, CA 94305.
Flatiron Bio, Palo Alto, CA 94301.
Proc Natl Acad Sci U S A. 2024 Aug 6;121(32):e2409676121. doi: 10.1073/pnas.2409676121. Epub 2024 Jul 29.
Fragment correlation mass spectrometry correlates ion pairs generated from the same fragmentation pathway, achieved by covariance mapping of tandem mass spectra generated with an unmodified linear ion trap without preseparation. We enable the identification of different precursors at different charge states in a complex mixture from a large isolation window, empowering an analytical approach for data-independent acquisition. The method resolves and matches isobaric fragments, internal ions, and disulfide bond fragments. We suggest that this method represents a major advance for analyzing structures of biopolymers in mixtures.
片段关联质谱法通过对未经修饰的线性离子阱产生的串联质谱进行协方差映射，而无需预分离，将同一碎裂途径产生的离子对进行关联。我们能够从大的隔离窗口中识别复杂混合物中不同电荷状态的不同前体，从而实现一种用于数据非依赖采集的分析方法。该方法可解析并匹配等压碎片、内部离子和二硫键碎片。我们认为该方法是分析混合物中生物聚合物结构的一项重大进展。
