Asghari-Moghadam Morteza, Mehri Mehran
Department of Animal Sciences, Faculty of Agriculture, University of Zabol, Zabol, 98661-5538, Sistan, Iran.
Biol Trace Elem Res. 2025 Apr;203(4):2409-2419. doi: 10.1007/s12011-024-04323-0. Epub 2024 Jul 30.
This study aimed to investigate the impact of dietary selenium treatments on various sperm parameters and antioxidant responses in aged roosters to determine the relative bioavailability value (RBV) and optimize selenium supplementation. Over 40 days, starting from week 47 of age, the roosters were fed ten experimental diets, including a basal diet without selenium supplement and nine selenium-treated diets. These selenium-treated diets comprised three different selenium sources (selenium selenite: SS; SelPlex: Se-enriched yeast; selenium nanoparticles: SeNPs), each with three levels of selenium supplements (0.15, 0.30, and 0.45 mg/kg). Statistical analysis indicated significant treatment effects on all measured parameters except sperm volume. Sperm motility and viability increased linearly with higher dietary selenium levels. The relative bioavailability values (RBV) of SelPlex and SeNPs compared to SS were estimated using the slope ratio and exponential regression methods. Using the slope-ratio method, the RBV for sperm volume was 457% for SelPlex and 314% for SeNPs, compared to SS. Using exponential regression, the RBV of SelPlex and SeNPs for various parameters were as follows: for MDA (malondialdehyde), 260% and 317%; for TAC (total antioxidant capacity), 447% and 294%; for sperm morphology, 227% and 423%; and for sperm concentration, 346% and 298%, respectively. Principal component analysis revealed strong correlations between sperm motility, viability, and antioxidant parameters, with weaker associations observed between sperm volume and testosterone. Optimization using a desirability function identified 0.45 mg/kg selenium supplementation using SeNPs as optimal, maximizing sperm parameters and antioxidant responses while minimizing MDA and morphology. These findings offer valuable insights into effective selenium supplementation strategies to enhance avian reproductive health in aged roosters.
本研究旨在探讨日粮硒处理对老龄公鸡各项精子参数及抗氧化反应的影响，以确定相对生物利用率值（RBV）并优化硒补充量。从47周龄开始，在40天的时间里，给公鸡饲喂10种试验日粮，包括一种不添加硒的基础日粮和9种经硒处理的日粮。这些经硒处理的日粮包含三种不同的硒源（亚硒酸钠：SS；SelPlex：富硒酵母；硒纳米颗粒：SeNPs），每种硒源有三个水平的硒补充量（0.15、0.30和0.45毫克/千克）。统计分析表明，除精液体积外，所有测量参数均有显著的处理效应。精子活力和存活率随日粮硒水平的升高呈线性增加。使用斜率比法和指数回归法估计了SelPlex和SeNPs相对于SS的相对生物利用率值（RBV）。使用斜率比法，与SS相比，SelPlex的精液体积RBV为457%，SeNPs为314%。使用指数回归法，SelPlex和SeNPs在各种参数上的RBV如下：丙二醛（MDA）为260%和317%；总抗氧化能力（TAC）为447%和294%；精子形态为227%和423%；精子浓度分别为346%和298%。主成分分析显示精子活力、存活率与抗氧化参数之间有很强的相关性，而精液体积与睾酮之间的相关性较弱。使用合意性函数进行优化，确定以0.45毫克/千克的硒纳米颗粒进行硒补充为最佳，可使精子参数和抗氧化反应最大化，同时使丙二醛和形态最小化。这些发现为提高老龄公鸡禽类生殖健康的有效硒补充策略提供了有价值的见解。
