State Public Health Laboratory, Directorate of Public Health and Preventive Medicine, DMS Campus, Teynampet, Chennai, Tamil Nadu, 600 006, India.
Department of Microbiology, Centre for Infectious Diseases, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, 600 077, India.
Sci Rep. 2024 Jul 30;14(1):17476. doi: 10.1038/s41598-024-68678-z.
In December 2023, we observed a notable shift in the COVID-19 landscape, when JN.1 omicron emerged as the predominant SARS-CoV-2 variant with a 95% incidence. We characterized the clinical profile, and genetic changes in JN.1, an emerging SARS-CoV-2 variant of interest. Whole genome sequencing was performed on SARS-CoV-2 positive clinical specimens, followed by sequence analysis. Mutations within the spike protein sequences were analysed and compared with the previously reported lineages and sub-lineages, to identify the potential impact of the unique mutations on protein structure and possible alterations in the functionality. Several unique and dynamic mutations were identified herein. Molecular docking analysis showed changes in the binding affinity, and key interacting residues of wild-type and mutated structures with key host cell receptors of SARS-CoV-2 entry viz., ACE2, CD147, CD209L and AXL. Our data provides key insights on the emergence of newer variants and highlights the necessity for robust and sustained global genomic surveillance of SARS-CoV-2.
2023 年 12 月，我们观察到 COVID-19 形势发生了显著变化，当时 JN.1 奥密克戎成为主要的 SARS-CoV-2 变体，发病率为 95%。我们对 JN.1（一种新兴的 SARS-CoV-2 关注变体）的临床特征和遗传变化进行了特征描述。对 SARS-CoV-2 阳性临床标本进行了全基因组测序，然后进行序列分析。分析了刺突蛋白序列内的突变，并与先前报道的谱系和亚谱系进行了比较，以确定独特突变对蛋白质结构的潜在影响以及功能可能发生的改变。本文鉴定了几个独特和动态的突变。分子对接分析显示，结合亲和力发生了变化，野生型和突变型结构与 SARS-CoV-2 进入的关键宿主细胞受体 ACE2、CD147、CD209L 和 AXL 的关键相互作用残基也发生了变化。我们的数据提供了有关新变体出现的关键见解，并强调了对 SARS-CoV-2 进行强大和持续的全球基因组监测的必要性。
