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健康小鼠中表面修饰的 G1 和 G4 PAMAM 树枝状聚合物的单侧给药，以评估树枝状聚合物在大脑中的迁移。

Unilateral Administration of Surface-Modified G1 and G4 PAMAM Dendrimers in Healthy Mice to Assess Dendrimer Migration in the Brain.

机构信息

College of Medicine, Central Michigan University, Mount Pleasant, Michigan 48859, United States.

Program of Neuroscience, Central Michigan University, Mount Pleasant, Michigan 48859, United States.

出版信息

ACS Appl Mater Interfaces. 2024 Aug 14;16(32):41907-41915. doi: 10.1021/acsami.4c09137. Epub 2024 Jul 31.

DOI:10.1021/acsami.4c09137
PMID:39083440
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11331434/
Abstract

Polyamidoamine (PAMAM) dendrimers are nanoparticles that have a wide scope in the field of biomedicine. Previous evidence shows that the generation 4 (G4) dendrimers with a 100% amine surface (G4-NH) are highly toxic to cells and due to their positively charged amine groups. To reduce the toxicity, we modified the surface of the dendrimers to have more neutral functional groups, with 10% of the surface covered with -NH and 90% of the surface covered with hydroxyl groups (-OH; G4-90/10). Our previous data show that these modified dendrimers are taken up by cells, neurons, and different types of stem cells and neurons and glial cells . The toxicity assay shows that these modified dendrimers are less toxic compared with G4-NH2 dendrimers. Moreover, prolonged dendrimer exposure (G1-90/10 and G4-90/10), up to 3 weeks following unilateral intrastriatal injections into the striatum of mice, showed that dendrimers have the tendency to migrate within the brain via corpus callosum at different rates depending on their size. We also found that there is a difference in migration between the G1 and G4 dendrimers based on their size differences. The G4 dendrimers migrate in the anterior and posterior directions as well as more laterally from the site of injection in the striatum compared to the G1 dendrimers. Moreover, the G4 dendrimers have unique projections from the site of injection to the cortical areas.

摘要

聚酰胺-胺（PAMAM）树枝状聚合物是一类具有广泛应用前景的纳米粒子，在生物医药领域有广泛的应用。已有证据表明，具有 100%胺表面的第 4 代（G4）树枝状聚合物（G4-NH）对细胞具有高度毒性，这主要是由于其带正电荷的胺基。为了降低毒性，我们对树枝状聚合物的表面进行了修饰，使其具有更多中性的官能团，其中 10%的表面被 -NH 覆盖，90%的表面被 -OH 覆盖（G4-90/10）。我们之前的数据表明，这些修饰后的树枝状聚合物被细胞、神经元和不同类型的干细胞摄取，以及神经元和神经胶质细胞。毒性测定表明，与 G4-NH2 树枝状聚合物相比，这些修饰后的树枝状聚合物的毒性较低。此外，延长树枝状聚合物暴露时间（G1-90/10 和 G4-90/10），在单侧纹状体注射到小鼠纹状体 3 周后，发现树枝状聚合物具有通过胼胝体在大脑内迁移的趋势，其迁移速度取决于其大小。我们还发现，基于大小差异，G1 和 G4 树枝状聚合物之间的迁移存在差异。与 G1 树枝状聚合物相比，G4 树枝状聚合物在纹状体注射部位的前、后和侧向迁移更为明显。此外，G4 树枝状聚合物在从注射部位到皮质区域的迁移过程中有独特的突起。

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本文引用的文献

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Mol Pharm. 2024 Jan 1;21(1):333-345. doi: 10.1021/acs.molpharmaceut.3c00880. Epub 2023 Dec 7.
2
Insights into nanoparticles-induced neurotoxicity and cope up strategies.纳米颗粒诱导的神经毒性及应对策略的见解
Front Neurosci. 2023 May 4;17:1127460. doi: 10.3389/fnins.2023.1127460. eCollection 2023.
3
Common mechanisms underlying axonal transport deficits in neurodegenerative diseases: a mini review.
神经退行性疾病中轴突运输缺陷的常见潜在机制：一篇综述短文
Front Mol Neurosci. 2023 Apr 24;16:1172197. doi: 10.3389/fnmol.2023.1172197. eCollection 2023.
4
Nanoparticles for Targeted Brain Drug Delivery: What Do We Know?用于靶向脑内药物递送的纳米颗粒：我们了解多少？
Int J Mol Sci. 2021 Oct 28;22(21):11654. doi: 10.3390/ijms222111654.
5
Reduced coupling between cerebrospinal fluid flow and global brain activity is linked to Alzheimer disease-related pathology.脑脊液流动与全脑活动之间的耦合减弱与阿尔茨海默病相关病理有关。
PLoS Biol. 2021 Jun 1;19(6):e3001233. doi: 10.1371/journal.pbio.3001233. eCollection 2021 Jun.
6
A Mixed-Surface Polyamidoamine Dendrimer for In Vitro and In Vivo Delivery of Large Plasmids.一种用于大质粒体外和体内递送的混合表面聚酰胺-胺树枝状大分子。
Pharmaceutics. 2020 Jul 3;12(7):619. doi: 10.3390/pharmaceutics12070619.
7
Nanocarriers as Potential Drug Delivery Candidates for Overcoming the Blood-Brain Barrier: Challenges and Possibilities.纳米载体作为克服血脑屏障的潜在药物递送候选物：挑战与可能性
ACS Omega. 2020 Jun 1;5(22):12583-12595. doi: 10.1021/acsomega.0c01592. eCollection 2020 Jun 9.
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9
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Pharmaceutics. 2018 Dec 11;10(4):269. doi: 10.3390/pharmaceutics10040269.
10
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