National Key Laboratory for Tropical Crop Breeding, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
Agricultural Research Service, Natural Products Utilization Research Unit, U.S. Department of Agriculture, University of Mississippi, University, Mississippi 38677, United States.
J Agric Food Chem. 2024 Aug 14;72(32):17890-17902. doi: 10.1021/acs.jafc.4c04077. Epub 2024 Jul 31.
Banana wilt caused by f. sp. ( TR4) is the most destructive soil-borne fungal disease. Until now, there has been a lack of effective measures to control the disease. It is urgent to explore biocontrol agents to control TR4 and the secretion of mycotoxin. In this study, fluvirucin B6 was screened from using an activity-guided method. Fluvirucin B6 exhibited strong antifungal activity against TR4 (0.084 mM of EC value) and significantly inhibited mycelial growth and spore germination. Further studies demonstrated that fluvirucin B6 could cause the functional loss of mitochondria, the disorder of metabolism of TR4 cells, and the decrease of enzyme activities in the tricarboxylic acid cycle and electron transport chain, ultimately inhibiting mycotoxin metabolism. In a pot experiment, the application of fluvirucin B6 significantly decreased the incidence of banana wilt and the amount of TR4 and controlled fungal toxins in the soil. Additionally, fluvirucin B6 could positively regulate the changes in the structure of the banana rhizosphere microbial community, significantly enriching beneficial microbes associated with disease resistance. In summary, this study identifies fluvirucin B6, which plays versatile roles in managing fungal diseases and mycotoxins.
由 f. sp. (TR4)引起的香蕉枯萎病是最具破坏性的土传真菌病害。到目前为止，还缺乏有效的措施来控制这种疾病。迫切需要探索生物防治剂来控制 TR4 和真菌毒素的分泌。在这项研究中，使用活性导向法从 中筛选出了氟维菌素 B6。氟维菌素 B6 对 TR4 表现出很强的抗真菌活性（EC 值为 0.084mM），并显著抑制菌丝生长和孢子萌发。进一步的研究表明，氟维菌素 B6 可以导致线粒体功能丧失、TR4 细胞代谢紊乱以及三羧酸循环和电子传递链中酶活性降低，最终抑制真菌毒素代谢。在盆栽试验中，氟维菌素 B6 的应用显著降低了香蕉枯萎病的发病率和 TR4 的数量，并控制了土壤中的真菌毒素。此外，氟维菌素 B6 可以积极调节香蕉根际微生物群落的结构变化，显著富集与抗病相关的有益微生物。总之，这项研究确定了氟维菌素 B6 在管理真菌病害和真菌毒素方面具有多种作用。
