Department of Life Sciences, Central University of Jharkhand, Jharkhand, Ranchi, 835 222, India.
World J Microbiol Biotechnol. 2024 Aug 2;40(9):287. doi: 10.1007/s11274-024-04093-w.
Bacteriocins are antimicrobial peptides produced by bacteria to prevent the growth of pathogens. Combining bacteriocins with metal nanoparticles, like silver nanoparticles (AgNPs), has developed into a viable strategy to get over bacteriocin limitations. In this study, bacteriocin BacZY05 was extracted from Bacillus subtilis ZY05 and purified using various techniques. The resulting purified bacteriocin was then combined with silver nanoparticles to form bacteriocin silver nanoconjugates (BacZY05-AgNPs). The physicochemical properties of the BacZY05-AgNPs were characterized using various analytical techniques. The mean diameter of the synthesized AgNPs was approximately 20-60 nm with an oval or spherical shape. The antimicrobial activity of the BacZY05-AgNPs was evaluated against several indicator strains by their zone of inhibition (ZOI), using the agar well diffusion method. Compared to bacteriocin (ZOI- 13 to 20 mm) and AgNPs (ZOI- 10-22 mm) alone, the antibacterial activity data demonstrated a 1.3-1.5-fold increase in the activity of bacteriocin-nanoconjugates (ZOI- 22 to 26 mm). For Staphylococcus aureus MTCC3103 and Klebsiella pneumoniae MTCC109, BacZY05-capped AgNPs exhibited the lowest minimum inhibitory concentration (MIC), measuring 10.93 µg/mL. For Salmonella typhi NCIM2501, the MIC was 28.75 µg/mL. The highest MIC value was 57.5 µg/mL for Escherichia coli DH5α and Vibrio cholerae MTCC3909. With BacZY05-capped AgNPs, the lowest minimum bactericidal concentration (MBC) of 28.75 µg/mL was observed for Staphylococcus aureus MTCC31003. In the cases of Salmonella typhi NCIM2501 and Klebsiella pneumoniae MTCC109 concentration was 57.5 µg/mL. Vibrio cholerae MTCC3909 and Escherichia coli DH5α had the highest MBC values at 115 µg/mL.
细菌素是细菌产生的抗菌肽，用于防止病原体生长。将细菌素与金属纳米颗粒（如银纳米颗粒）结合已成为克服细菌素限制的可行策略。在这项研究中，从枯草芽孢杆菌 ZY05 中提取了细菌素 BacZY05，并使用各种技术进行了纯化。然后将得到的纯化细菌素与银纳米颗粒结合形成细菌素银纳米缀合物（BacZY05-AgNPs）。使用各种分析技术对 BacZY05-AgNPs 的物理化学性质进行了表征。合成的 AgNPs 的平均直径约为 20-60nm，呈椭圆形或球形。使用琼脂孔扩散法通过抑菌圈（ZOI）评估 BacZY05-AgNPs 对几种指示菌株的抗菌活性。与单独的细菌素（ZOI-13 至 20mm）和 AgNPs（ZOI-10-22mm）相比，抗菌活性数据表明细菌素-纳米缀合物的活性增加了 1.3-1.5 倍（ZOI-22 至 26mm）。对于金黄色葡萄球菌 MTCC3103 和肺炎克雷伯菌 MTCC109，BacZY05 封端的 AgNPs 表现出最低的最小抑菌浓度（MIC），为 10.93µg/mL。对于伤寒沙门氏菌 NCIM2501，MIC 为 28.75µg/mL。对于大肠杆菌 DH5α 和霍乱弧菌 MTCC3909，MIC 值最高为 57.5µg/mL。用 BacZY05 封端的 AgNPs，金黄色葡萄球菌 MTCC31003 的最低最小杀菌浓度（MBC）为 28.75µg/mL。在伤寒沙门氏菌 NCIM2501 和肺炎克雷伯菌 MTCC109 的情况下，浓度为 57.5µg/mL。霍乱弧菌 MTCC3909 和大肠杆菌 DH5α 的 MBC 值最高，为 115µg/mL。
