Saqib Saddam, Nazeer Amna, Ali Muhammad, Zaman Wajid, Younas Muhammad, Shahzad Asim, Nisar Momina
Department of Biotechnology, Mohi-Ud-Din Islamic University, Nerian Sharif, 12080, AJ&K, Pakistan.
State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
Biometals. 2022 Oct;35(5):967-985. doi: 10.1007/s10534-022-00417-1. Epub 2022 Jul 14.
Recent advances fascinated the use of biological resources in combination with metals to obtain high quality biometals and apply its advantages in different fields. Endophytic mediated Zinc oxide nanoparticles (ZnO-NPs) is an economical and ecofriendly way for farmers to avoid Zn deficiency in rice crop and obtain high yield. Here we synthesized ZnO-NPs utilizing endophytic bacterial strain of Enterobacter hormaechei (E. hormaechei). The physiochemical properties of the prepared NPs were determined through UV-Vis spectroscopy, XRD, FT-IR, SEM and TEM. The prepared NPs revealed surface plasmon resonance (SPR) at 320 nm (nm) and crystalline structure with 21 nm average crystalline size. FT-IR spectra showed the presence of carboxylic, alcohol and amine functional groups, which confirm the biometallic assembling of the ZnO and endophytic bacterial functional groups. SEM showed pyramidal symmetry whereas TEM revealed poly dispersed spherical shape with particle size distribution 18-48 nm. Our results showed that prepared NPs possess significant antifungal, antibacterial and antioxidant potential at 25, 50 and 100 µg/mL concentrations. Moreover, Cytotoxic and hemolytic assay showed significant results (less % viability and hemolysis activity) at 50 and 100 µg/mL (ZnO-NP's) concentrations as compared to control. The prepared ZnO-NPs were used as bio fertilizer in various concentrations as a foliar spray, which showed significant enhancement of the rice plant growth, along with chlorophyll, proteins and carotenoid contents. These results recommend that endophytic mediated ZnO-NPs are biocompatible and possess significant potential for agricultural applications.
最近的进展使生物资源与金属结合使用以获得高质量生物金属并将其优势应用于不同领域的方法备受关注。内生菌介导的氧化锌纳米颗粒（ZnO-NPs）是一种经济且环保的方式，可帮助农民避免水稻作物缺锌并实现高产。在此，我们利用霍氏肠杆菌（E. hormaechei）的内生细菌菌株合成了ZnO-NPs。通过紫外可见光谱、X射线衍射、傅里叶变换红外光谱、扫描电子显微镜和透射电子显微镜测定了所制备纳米颗粒的物理化学性质。所制备的纳米颗粒在320纳米处显示出表面等离子体共振（SPR），并且具有平均晶体尺寸为21纳米的晶体结构。傅里叶变换红外光谱显示存在羧基、醇基和胺基官能团，这证实了ZnO与内生细菌官能团的生物金属组装。扫描电子显微镜显示出金字塔对称性，而透射电子显微镜显示出多分散的球形，粒径分布为18 - 48纳米。我们的结果表明，所制备的纳米颗粒在25、50和100微克/毫升浓度下具有显著的抗真菌、抗菌和抗氧化潜力。此外，细胞毒性和溶血试验表明，与对照组相比，在50和100微克/毫升（ZnO-NP）浓度下有显著结果（活力百分比和溶血活性较低）。所制备的ZnO-NPs以不同浓度作为叶面喷施用作生物肥料，这显示出水稻植株生长以及叶绿素、蛋白质和类胡萝卜素含量有显著提高。这些结果表明，内生菌介导的ZnO-NPs具有生物相容性，在农业应用中具有巨大潜力。
