Isnard Pierre, Humphreys Benjamin D
Division of Nephrology, Department of Medicine, Washington University in St. Louis, St. Louis, Missouri.
Division of Nephrology, Department of Medicine, Washington University in St. Louis, St. Louis, Missouri; Department of Developmental Biology, Washington University in St. Louis, St. Louis, Missouri.
Am J Pathol. 2025 Jan;195(1):23-39. doi: 10.1016/j.ajpath.2024.06.012. Epub 2024 Aug 5.
The recent arrival of high-resolution spatial transcriptomics (ST) technologies is generating a veritable revolution in life sciences, enabling biomolecules to be measured in their native spatial context. By integrating morphology and molecular biology, ST technologies offer the potential of improving the understanding of tissue biology and disease and may also provide meaningful clinical insights. This review describes the main ST technologies currently available and the computational analysis for data interpretation and visualization, and illustrate their scientific and potential medical interest in the context of kidney disease. Finally, we discuss the perspectives and challenges of these booming new technologies.
高分辨率空间转录组学（ST）技术的近期出现正在生命科学领域引发一场名副其实的革命，使生物分子能够在其原生空间背景下进行测量。通过整合形态学和分子生物学，ST技术提供了增进对组织生物学和疾病理解的潜力，还可能提供有意义的临床见解。本综述描述了当前可用的主要ST技术以及用于数据解释和可视化的计算分析，并阐述了它们在肾脏疾病背景下的科学及潜在医学价值。最后，我们讨论了这些蓬勃发展的新技术的前景和挑战。
