Cocks B G, Pyle L E, Finch L R
Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia.
Nucleic Acids Res. 1989 Aug 25;17(16):6713-9. doi: 10.1093/nar/17.16.6713.
A physical map is presented for the 900 kilobase pair genome of Ureaplasma urealyticum 960T, locating 29 sites for 6 restriction endonucleases. The large restriction fragments were separated and sized by pulsed-field agarose gel electrophoresis (PFGE). Their locations on the map were determined by probing Southern blots of digests with individual fragments isolated from other digests and by correlating the products of double digestions and partial digestions. An end-labelling technique was used to detect small fragments not readily observed by PFGE. Two loci for rRNA genes have been determined by probing with cloned DNA.
展示了解脲脲原体960T 900千碱基对基因组的物理图谱，定位了6种限制性内切酶的29个位点。通过脉冲场琼脂糖凝胶电泳（PFGE）分离并确定大的限制性片段的大小。通过用从其他酶切消化中分离出的单个片段探测酶切消化产物的Southern印迹，并通过关联双酶切消化和部分酶切消化的产物，确定它们在图谱上的位置。使用末端标记技术检测PFGE不易观察到的小片段。通过用克隆DNA探测确定了两个rRNA基因位点。
