• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

补体3b/补体3bi沉积不足及酵母调理作用缺陷。II. 一种能够纠正缺陷血清的调理素辅助因子的部分纯化及初步特性分析

Suboptimal C3b/C3bi deposition and defective yeast opsonization. II. Partial purification and preliminary characterization of an opsonic co-factor able to correct sera with the defect.

作者信息

Turner M W, Seymour N D, Kazatchkine M D, Mowbray J F

出版信息

Clin Exp Immunol. 1985 Nov;62(2):435-41.

PMID:3910314
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1577452/
Abstract

Using a correction assay a factor essential for normal deposition of C3 fragments on zymosan was identified in fractions of serum obtained by Sephacryl S-300 gel filtration (eluting between IgG and albumin), preparative Pevikon block electrophoresis (eluting in the beta-region) and DEAE-ion-exchange chromatography (eluting immediately post IgG). Active material was also identified in a commercial preparation of transferrin. A combination of DEAE-ion-exchange chromatography and gel filtration was used to partially purify the co-factor. The factor has an approximate relative molecular mass of 70,000-80,000.

摘要

通过校正试验，在经Sephacryl S - 300凝胶过滤（在IgG和白蛋白之间洗脱）、制备性Pevikon块电泳（在β区洗脱）和DEAE离子交换色谱（紧跟IgG后洗脱）获得的血清组分中，鉴定出一种对C3片段在酵母聚糖上正常沉积至关重要的因子。在一种商业转铁蛋白制剂中也鉴定出了活性物质。采用DEAE离子交换色谱和凝胶过滤相结合的方法对该辅因子进行了部分纯化

相似文献

1
Suboptimal C3b/C3bi deposition and defective yeast opsonization. II. Partial purification and preliminary characterization of an opsonic co-factor able to correct sera with the defect.补体3b/补体3bi沉积不足及酵母调理作用缺陷。II. 一种能够纠正缺陷血清的调理素辅助因子的部分纯化及初步特性分析
Clin Exp Immunol. 1985 Nov;62(2):435-41.
2
A study of C3b deposition on yeast surfaces by sera of known opsonic potential.一项关于已知调理素活性潜力的血清对酵母表面C3b沉积的研究。
Clin Exp Immunol. 1981 Nov;46(2):412-9.
3
Suboptimal C3b/C3bi deposition and defective yeast opsonization. I. Evidence for the absence of essential co-factor activity.补体3b/补体3bi沉积不足及酵母调理作用缺陷。I. 缺乏关键辅助因子活性的证据。
Clin Exp Immunol. 1985 Nov;62(2):427-34.
4
Evaluation of C3b/C3bi opsonization and chemiluminescence with selected yeasts and bacteria using sera of different opsonic potential.使用具有不同调理素活性的血清，对选定的酵母和细菌进行C3b/C3bi调理作用及化学发光的评估。
Immunology. 1986 May;58(1):111-5.
5
Opsonization of yeast by human serum IgA anti-mannan antibodies and phagocytosis by human polymorphonuclear leucocytes.人血清IgA抗甘露聚糖抗体对酵母的调理作用以及人多形核白细胞的吞噬作用。
Clin Exp Immunol. 1987 Apr;68(1):200-8.
6
Opsonization of yeast cells with equine iC3b, C3b, and IgG.用马的iC3b、C3b和IgG对酵母细胞进行调理作用。
Vet Immunol Immunopathol. 2001 Aug 10;80(3-4):209-23. doi: 10.1016/s0165-2427(01)00262-8.
7
The role of opsonins in vacuolar sealing and the ingestion of zymosan by human neutrophils.调理素在人中性粒细胞液泡封闭及酵母聚糖摄取中的作用。
Immunology. 1986 Sep;59(1):69-74.
8
Differences in the ingestion mechanisms of IgG and C3b particles in phagocytosis by neutrophils.中性粒细胞吞噬作用中IgG和C3b颗粒摄取机制的差异。
Immunology. 1982 Apr;45(4):727-36.
9
Isolation and characterization of rat complement factor B and its interaction with cell-bound human C3.大鼠补体因子B的分离、特性鉴定及其与细胞结合的人C3的相互作用
Immunology. 1980 Dec;41(4):849-55.
10
The rabbit properdin system: I. Identification of a new factor and purification of rabbit properdin.兔备解素系统：I. 一种新因子的鉴定及兔备解素的纯化。
J Immunol. 1980 Jun;124(6):2625-31.

引用本文的文献

1
Evaluation of C3b/C3bi opsonization and chemiluminescence with selected yeasts and bacteria using sera of different opsonic potential.使用具有不同调理素活性的血清，对选定的酵母和细菌进行C3b/C3bi调理作用及化学发光的评估。
Immunology. 1986 May;58(1):111-5.
2
New microbial and host factors in disseminated gonococcal infection: case report.播散性淋球菌感染中的新微生物和宿主因素：病例报告。
Genitourin Med. 1987 Jun;63(3):169-71. doi: 10.1136/sti.63.3.169.
3
Suboptimal C3b/C3bi deposition and defective yeast opsonization. I. Evidence for the absence of essential co-factor activity.补体3b/补体3bi沉积不足及酵母调理作用缺陷。I. 缺乏关键辅助因子活性的证据。
Clin Exp Immunol. 1985 Nov;62(2):427-34.
4
Protracted enteric cryptosporidial infection in selective immunoglobulin A and saccharomyces opsonin deficiencies.选择性免疫球蛋白A和酿酒酵母调理素缺乏症中的持续性肠道隐孢子虫感染
Gut. 1990 Jun;31(6):714-6. doi: 10.1136/gut.31.6.714.

本文引用的文献

1
The properdin system and immunity. I. Demonstration and isolation of a new serum protein, properdin, and its role in immune phenomena.备解素系统与免疫。I. 一种新的血清蛋白——备解素的证实、分离及其在免疫现象中的作用。
Science. 1954 Aug 20;120(3112):279-85. doi: 10.1126/science.120.3112.279.
2
The role of immunoglobulins in alternative pathway activation by zymosan. II. The effect of IgG on the kinetics of the alternative pathway.免疫球蛋白在酵母聚糖激活替代途径中的作用。II. IgG对替代途径动力学的影响。
J Immunol. 1981 Jan;126(1):11-5.
3
The effect of C3 levels on yeast opsonization by normal and pathological sera: identification of a complement independent opsonin.C3水平对正常和病理血清酵母调理作用的影响：补体非依赖性调理素的鉴定。
Clin Exp Immunol. 1983 Dec;54(3):793-800.
4
Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity.聚丙烯酰胺凝胶中蛋白质的银染：一种具有增强均匀灵敏度的改良方法。
Anal Biochem. 1981 Nov 1;117(2):307-10. doi: 10.1016/0003-2697(81)90783-1.
5
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
6
Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels.通过SDS-聚丙烯酰胺凝胶电泳对多肽链进行分子量估计
Biochem Biophys Res Commun. 1967 Sep 7;28(5):815-20. doi: 10.1016/0006-291x(67)90391-9.
7
Alternate complement pathway: factors involved in cobra venom factor (CoVF) activation of the third component of complement (C3).替代补体途径：参与眼镜蛇毒因子（CoVF）激活补体第三成分（C3）的相关因子。
J Immunol. 1973 Jan;110(1):128-38.
8
Suboptimal C3b/C3bi deposition and defective yeast opsonization. I. Evidence for the absence of essential co-factor activity.补体3b/补体3bi沉积不足及酵母调理作用缺陷。I. 缺乏关键辅助因子活性的证据。
Clin Exp Immunol. 1985 Nov;62(2):427-34.
9
Properdin: binding to C3b and stabilization of the C3b-dependent C3 convertase.备解素：与C3b结合并稳定依赖C3b的C3转化酶。
J Exp Med. 1975 Oct 1;142(4):856-63. doi: 10.1084/jem.142.4.856.
10
A phagocytosis-enhancing factor in human plasma.人类血浆中的一种吞噬作用增强因子。
Immunology. 1976 Jun;30(6):915-24.