一项关于已知调理素活性潜力的血清对酵母表面C3b沉积的研究。
A study of C3b deposition on yeast surfaces by sera of known opsonic potential.
作者信息
Turner M W, Mowbray J F, Roberton D R
出版信息
Clin Exp Immunol. 1981 Nov;46(2):412-9.
Abstract
C3c fragments released by the actin of trypsin from C3b molecules bound to zymosan may be readily quantitated by conventional gel techniques to give a measurement of the efficiency of C3b opsonization. Using this approach, the rate of deposition of C3b molecules was found to be very rapid in sera known to opsonize yeast normally. In contrast, sera defective in yeast opsonization deposited C3b much more slowly. The C3b elution technique was found to correlate well with both a direct phagocytosis assay using baker's yeast (r = 0.87, P less than 0.001) and a recently described neutrophil iodide uptake assay (r = 0.88, P less than 0.001).
摘要
用胰蛋白酶作用于与酵母聚糖结合的C3b分子，使其释放出C3c片段，通过传统凝胶技术可轻松对其进行定量，从而测定C3b调理作用的效率。采用这种方法发现，在已知能正常调理酵母的血清中，C3b分子的沉积速率非常快。相比之下，酵母调理作用存在缺陷的血清中C3b的沉积要慢得多。结果发现，C3b洗脱技术与使用面包酵母的直接吞噬试验（r = 0.87，P小于0.001）以及最近描述的中性粒细胞碘摄取试验（r = 0.88，P小于0.001）都具有很好的相关性。
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