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使用具有不同调理素活性的血清,对选定的酵母和细菌进行C3b/C3bi调理作用及化学发光的评估。

Evaluation of C3b/C3bi opsonization and chemiluminescence with selected yeasts and bacteria using sera of different opsonic potential.

作者信息

Turner M W, Grant C, Seymour N D, Harvey B, Levinsky R J

出版信息

Immunology. 1986 May;58(1):111-5.

Abstract

C3 fragment opsonization and chemiluminescence were assessed using two yeasts (Saccharomyces cerevisiae and Candida albicans) and two strains of bacteria (Staphylococcus aureus and Escherichia coli) following incubation with sera previously shown to be either efficient or poor in the opsonization of baker's yeast. Sera with poor function consistently fixed lower levels of C3 fragments and generated less chemiluminescence with all four organisms than sera with normal function. The differences were most marked with the two yeasts. We conclude that sera with normal complement function but manifesting a relatively common defect in the opsonization of baker's yeast have a general opsonic defect.

摘要

使用两种酵母(酿酒酵母和白色念珠菌)和两种细菌菌株(金黄色葡萄球菌和大肠杆菌),在与先前已证明对面包酵母调理作用高效或低效的血清孵育后,评估C3片段调理作用和化学发光。与正常功能的血清相比,功能较差的血清在所有四种生物体上始终固定较低水平的C3片段,并产生较少的化学发光。在两种酵母中差异最为明显。我们得出结论,具有正常补体功能但在面包酵母调理作用中表现出相对常见缺陷的血清存在普遍的调理缺陷。

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