Yan Weifeng, Deng Shengyang, Guo Xiaoyun, Li Rui
Jinhua Maternal and Child Health Hospital, Jinhua, 321000, China.
J Fluoresc. 2025 Jun 2. doi: 10.1007/s10895-025-04389-6.
Herein, a fluorescent probe named IsoIm-SF with the isovanillin-containing imidazo backbone was developed for detecting sulfite in cellular pulmonary ventilation protection condition. In solution, the established pulmonary ventilation protection condition suggested no obvious impact on the detection. IsoIm-SF exhibited high sensitivity, long linear range, high steadiness, rapid response, high selectivity, and low cytotoxicity. In pulmonary cells, IsoIm-SF realized the imaging of both the exogenous and endogenous sulfite. Further, IsoIm-SF might monitor the sulfite level to indicate the pulmonary ventilation protection efficacy, including that contributed by the anesthetic treatment. This work was a challenging trial for establishing the anesthetic-related therapeutics to improve the methodology of the pulmonary ventilation protection.
在此，开发了一种名为IsoIm-SF的荧光探针，其具有含异香草醛的咪唑骨架，用于在细胞肺通气保护条件下检测亚硫酸盐。在溶液中，所建立的肺通气保护条件表明对检测没有明显影响。IsoIm-SF表现出高灵敏度、长线性范围、高稳定性、快速响应、高选择性和低细胞毒性。在肺细胞中，IsoIm-SF实现了外源性和内源性亚硫酸盐的成像。此外，IsoIm-SF可以监测亚硫酸盐水平以指示肺通气保护效果，包括麻醉治疗所带来的效果。这项工作是建立麻醉相关治疗方法以改进肺通气保护方法的一项具有挑战性的试验。
