Chen Xiaohang, Huang Liyu, Zhang Mengyuan, Lin Shuoqi, Xie Jing, Li Hengyi, Wang Xing, Lu Youguang, Zheng Dali
Fujian Key Laboratory of Oral Diseases, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, China.
Department of Preventive Dentistry, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, China.
Front Pharmacol. 2024 Jul 22;15:1423115. doi: 10.3389/fphar.2024.1423115. eCollection 2024.
Plant-derived nanovesicles have emerged as potential agents for combating tumors. In this study, we investigated the inhibitory effects of -derived nanovesicles (PnNVs) on the proliferation and migration of squamous cell carcinoma. Additionally, we explored the relationship between plant tuber size and the physical properties, composition and bioactivity of these nanovesicles.
We isolated PnNVs from tubers of varying sizes: small-sized (s_PnNVs), medium-sized (m_PnNVs) and large-sized (l_PnNVs), and evaluated for size, potential, and morphology. Cellular uptake efficiency was assessed using confocal microscopy and flow cytometry. The ability of different PnNVs to inhibit oral squamous cell carcinoma cells was evaluated using plate cloning, CCK8 assay, and scratch healing assay. Off-target metabolomics was used to compare metabolic compounds of different PnNVs.
Our findings revealed that s_PnNVs exhibited lower potential but had the highest cellular uptake efficiency, whereas m_PnNVs were characterized by the smallest size and lowest cellular uptake efficiency. Notably, m_PnNVs demonstrated the most effective inhibition of squamous cell carcinoma growth and migration. Compositional analyses showed that PnNVs were rich in proteins and contained lower levels of RNA, with l_PnNVs having the highest protein content. Furthermore, untargeted metabolomics analysis revealed a significant increase in the expression of specific antitumour-related metabolites in m_PnNVs compared to s_PnNVs and l_PnNVs.
Overall, our results underscore the influence of plant tuber size on the bioactivity of the nanovesicles from which they are derived, emphasizing its importance for experimental design and study reproducibility.
植物来源的纳米囊泡已成为对抗肿瘤的潜在药物。在本研究中，我们研究了植物来源的纳米囊泡（PnNVs）对鳞状细胞癌增殖和迁移的抑制作用。此外，我们还探讨了植物块茎大小与这些纳米囊泡的物理性质、组成和生物活性之间的关系。
我们从不同大小的块茎中分离出PnNVs：小尺寸（s_PnNVs）、中尺寸（m_PnNVs）和大尺寸（l_PnNVs），并对其大小、电位和形态进行了评估。使用共聚焦显微镜和流式细胞术评估细胞摄取效率。使用平板克隆、CCK8测定和划痕愈合试验评估不同PnNVs抑制口腔鳞状细胞癌细胞的能力。采用非靶向代谢组学比较不同PnNVs的代谢化合物。
我们的研究结果表明，s_PnNVs的电位较低，但细胞摄取效率最高，而m_PnNVs的特点是尺寸最小，细胞摄取效率最低。值得注意的是，m_PnNVs对鳞状细胞癌的生长和迁移具有最有效的抑制作用。成分分析表明，PnNVs富含蛋白质，RNA含量较低，l_PnNVs的蛋白质含量最高。此外，非靶向代谢组学分析显示，与s_PnNVs和l_PnNVs相比，m_PnNVs中特定抗肿瘤相关代谢物的表达显著增加。
总体而言，我们的结果强调了植物块茎大小对其来源的纳米囊泡生物活性的影响，强调了其对实验设计和研究可重复性的重要性。
