Pavlinkova Gabriela, Smolik Ondrej
Laboratory of Molecular Pathogenetics, Institute of Biotechnology Czech Academy of Sciences, Vestec, Czechia.
Front Cell Dev Biol. 2024 Jul 22;12:1435546. doi: 10.3389/fcell.2024.1435546. eCollection 2024.
Transcription factors belonging to the basic helix-loop-helix (bHLH) family are key regulators of cell fate specification and differentiation during development. Their dysregulation is implicated not only in developmental abnormalities but also in various adult diseases and cancers. Recently, the abilities of bHLH factors have been exploited in reprogramming strategies for cell replacement therapy. One such factor is NEUROD1, which has been associated with the reprogramming of the epigenetic landscape and potentially possessing pioneer factor abilities, initiating neuronal developmental programs, and enforcing pancreatic endocrine differentiation. The review aims to consolidate current knowledge on NEUROD1's multifaceted roles and mechanistic pathways in human and mouse cell differentiation and reprogramming, exploring NEUROD1 roles in guiding the development and reprogramming of neuroendocrine cell lineages. The review focuses on NEUROD1's molecular mechanisms, its interactions with other transcription factors, its role as a pioneer factor in chromatin remodeling, and its potential in cell reprogramming. We also show a differential potential of NEUROD1 in differentiation of neurons and pancreatic endocrine cells, highlighting its therapeutic potential and the necessity for further research to fully understand and utilize its capabilities.
属于基本螺旋-环-螺旋（bHLH）家族的转录因子是发育过程中细胞命运决定和分化的关键调节因子。它们的失调不仅与发育异常有关，还与各种成人疾病和癌症有关。最近，bHLH因子的能力已被用于细胞替代疗法的重编程策略中。其中一个这样的因子是NEUROD1，它与表观遗传景观的重编程有关，并可能具有先驱因子的能力，启动神经元发育程序，并促进胰腺内分泌分化。这篇综述旨在巩固目前关于NEUROD1在人类和小鼠细胞分化及重编程中的多方面作用和机制途径的知识，探索NEUROD1在指导神经内分泌细胞谱系的发育和重编程中的作用。该综述聚焦于NEUROD1的分子机制、它与其他转录因子的相互作用、它作为染色质重塑中的先驱因子的作用以及它在细胞重编程中的潜力。我们还展示了NEUROD1在神经元和胰腺内分泌细胞分化中的不同潜力，强调了其治疗潜力以及进一步研究以充分理解和利用其能力的必要性。
