Liesi P
J Histochem Cytochem. 1983 Jun;31(6):755-64. doi: 10.1177/31.6.6841971.
Laminin was localized in cultured mouse C1300 neuroblastoma cells by applying the peroxidase-antiperoxidase technique in preembedding electron microscopy. The results were compared to those obtained by indirect immunofluorescence and by the colloidal gold second antibody method on Epon-embedded ultrathin sections. Laminin was found in the cell membranes and within the rough endoplasmic reticulum as well as in intracytoplasmic vacuoles. Plasma membranes of the neuroblastoma cells showed a patchy localization of laminin that was apparently involved in cell-to-substrate attachment and in gap junction-like intercellular connections. Under normal conditions, the Golgi cisternae contained no laminin. Pretreatment of cells with micromolar concentrations of monensin, however, lead to an accumulation of laminin within the Golgi cisternae. These results support a role for laminin as an adhesion protein in cultured neuroblastoma cells and indicate that laminin is transported through the Golgi complex.
通过在包埋前电子显微镜中应用过氧化物酶-抗过氧化物酶技术，将层粘连蛋白定位在培养的小鼠C1300神经母细胞瘤细胞中。将结果与通过间接免疫荧光以及在Epon包埋的超薄切片上用胶体金二抗法获得的结果进行比较。在细胞膜、粗面内质网以及胞质空泡中发现了层粘连蛋白。神经母细胞瘤细胞的质膜显示出层粘连蛋白的斑片状定位，这显然参与了细胞与底物的附着以及间隙连接样的细胞间连接。在正常情况下，高尔基池不含层粘连蛋白。然而，用微摩尔浓度的莫能菌素预处理细胞会导致层粘连蛋白在高尔基池中积累。这些结果支持层粘连蛋白在培养的神经母细胞瘤细胞中作为一种粘附蛋白的作用，并表明层粘连蛋白通过高尔基复合体运输。
