Nunes Mattos Gabriela, Pessanha de Araújo Santiago Manuela Cristina, Sampaio Doria Chaves Ana Carolina, Rosenthal Amauri, Valeriano Tonon Renata, Correa Cabral Lourdes Maria
Programa de Pós-Graduação em Ciência de Alimentos, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-909, RJ, Brazil.
Embrapa Agroindústria de Alimentos, Av. das Américas, 29501, Rio de Janeiro 23020-470, RJ, Brazil.
Foods. 2022 Mar 20;11(6):885. doi: 10.3390/foods11060885.
This study evaluated the effect of different extraction technologies and conditions in order to obtain jaboticaba skin extracts. Firstly, the skins were extracted by conventional extraction, according to a rotatable central composite design, varying ethanol concentration, solid:liquid ratio, and temperature. Next, ultrasound-assisted extraction was performed using different power densities and times. Finally, high-pressure extractions were performed with varying pressures and times. For agitated bed extraction, the highest anthocyanin content was observed for ethanol concentrations varying between 60% and 80%. Thus, the independent variables which more influenced anthocyanin content were ethanol concentration and solid:liquid ratio. Folin-Ciocalteu reducing capacity was linearly affected by the increase in temperature. Ethanol concentration was the variable that most influenced ABTS+. On the other hand, the increase in ethanol concentration decreased the antioxidant capacity by ABTS+. Considering the ultrasound extraction, increasing its power did not affect total monomeric anthocyanins content, while the increase in process time had better yields. The highest antioxidant capacity and total monomeric anthocyanins were found for the highest extraction time. Similarly, with ultrasound, the increase in high hydrostatic-assisted extraction time positively influenced anthocyanin content and antioxidant capacity. As a result, the ultrasound-assisted method was found to be the best extraction technology for anthocyanins recovery.
本研究评估了不同提取技术和条件对获得巴西葡萄果皮提取物的影响。首先，根据旋转中心复合设计，通过常规提取法提取果皮，改变乙醇浓度、固液比和温度。接下来，使用不同的功率密度和时间进行超声辅助提取。最后，在不同压力和时间下进行高压提取。对于搅拌床提取，观察到乙醇浓度在60%至80%之间变化时花青素含量最高。因此，对花青素含量影响较大的自变量是乙醇浓度和固液比。福林-西奥尔特试剂还原能力受温度升高的线性影响。乙醇浓度是对ABTS+影响最大的变量。另一方面，乙醇浓度的增加降低了ABTS+的抗氧化能力。考虑到超声提取，提高其功率不会影响总单体花青素含量，而延长提取时间则产率更高。在最长提取时间时发现抗氧化能力和总单体花青素含量最高。同样，对于超声辅助提取，高静水压辅助提取时间的延长对花青素含量和抗氧化能力有积极影响。结果发现超声辅助方法是回收花青素的最佳提取技术。
