Institute of Tropical Medicine Tübingen, University Hospital Tübingen, Tübingen, Germany.
German Center for Infection Research Deutsches Zentrum für Infektionsforschung (DZIF), partner site Tübingen, Tübingen, Germany.
Front Cell Infect Microbiol. 2024 Jul 19;14:1405198. doi: 10.3389/fcimb.2024.1405198. eCollection 2024.
is the most common non-falciparum species in sub-Saharan Africa. Despite this, data on its genetic diversity is scarce. Therefore, we aimed to establish a genotyping approach based on size polymorphic regions that can be easily applied in molecular epidemiological studies.
Four potential genotyping markers, Pm02, Pm09, thrombospondin-related anonymous protein (pmtrap), and merozoite surface protein fragment 2 (pmmsp1 F2) were amplified via nested PCR and analysed using automated capillary gel electrophoresis.
We observed the highest allelic diversity for pmtrap (MOI = 1.61) and pmmsp1 F2 (He = 0.81). Further applying the two markers pmtrap and pmmsp1 F2 on a different sample set of 21 positive individuals followed up over one week, we saw a high consistency in their performance. The results show a large complexity and high dynamics of infections in the asymptomatic Gabonese study population.
We successfully implemented a new genotyping panel for consisting of only two markers: pmtrap and pmmsp1 F2. It can be easily applied in other settings to investigate the genotype diversity of populations, providing further important data on the molecular epidemiology of this parasite species.
是撒哈拉以南非洲最常见的非恶性疟原虫物种。尽管如此，关于其遗传多样性的数据仍然很少。因此，我们旨在建立一种基于大小多态性区域的基因分型方法，该方法可以很容易地应用于分子流行病学研究。
通过巢式 PCR 扩增了四个潜在的基因分型标记物，即 Pm02、Pm09、血小板反应蛋白相关匿名蛋白 (pmtrap) 和裂殖子表面蛋白片段 2 (pmmsp1 F2)，并使用自动毛细管凝胶电泳进行分析。
我们观察到 pmtrap（MOI = 1.61）和 pmmsp1 F2（He = 0.81）的等位基因多样性最高。进一步在一个为期一周的时间内对 21 个阳性个体的不同样本集上应用这两个标记物 pmtrap 和 pmmsp1 F2，我们看到它们的性能具有很高的一致性。结果表明，无症状加蓬研究人群中的 感染具有很大的复杂性和高度动态性。
我们成功地为 实施了一个新的基因分型面板，仅由两个标记物组成：pmtrap 和 pmmsp1 F2。它可以很容易地应用于其他环境中，以研究 种群的基因型多样性，为该寄生虫物种的分子流行病学提供进一步的重要数据。
