Rose John C, Belk Julia A, Wong Ivy Tsz-Lo, Luebeck Jens, Horn Hudson T, Daniel Bence, Jones Matthew G, Yost Kathryn E, Hung King L, Kolahi Kevin S, Curtis Ellis J, Kuo Calvin J, Bafna Vineet, Mischel Paul S, Chang Howard Y
Center for Personal Dynamic Regulomes, Stanford University, Stanford, California.
Sarafan ChEM-H, Stanford University, Stanford, California.
Cancer Discov. 2025 Jan 13;15(1):69-82. doi: 10.1158/2159-8290.CD-23-1117.
Our study harnesses a CRISPR-based method to examine ecDNA biogenesis, uncovering efficient circularization between double-strand breaks. ecDNAs and their corresponding chromosomal scars can form via nonhomologous end joining or microhomology-mediated end joining, but the ecDNA and scar formation processes are distinct. Based on our findings, we establish a mechanistic model of excisional ecDNA formation.
我们的研究利用一种基于CRISPR的方法来研究染色体外DNA（ecDNA）的生物发生，揭示了双链断裂之间的高效环化。ecDNA及其相应的染色体疤痕可通过非同源末端连接或微同源性介导的末端连接形成，但ecDNA和疤痕形成过程是不同的。基于我们的发现，我们建立了一个切除性ecDNA形成的机制模型。
