Qiu Quanpeng, Ding Yi, Guo Xiaolong, Han Jing, Zhang Jiaqi, Liu Yaping, She Junjun, Chen Yinnan
Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
Department of High Talent, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
Mol Med. 2025 Apr 1;31(1):123. doi: 10.1186/s10020-025-01164-y.
Extrachromosomal circular DNA (eccDNA) has potential in tumor diagnosis, particularly for improving diagnostic accuracy and early cancer detection; however, many challenges remain in its application to clinical practice.
We conducted a Circle-Seq analysis on clinical samples at different stages of colorectal cancer progression to examine the dynamic changes of eccDNA during the progression of colorectal cancer. We used breakpoint-specific PCR to verify candidate eccDNAs identified by Circle-Seq. The results were further validated using the AOM/DSS-induced colorectal cancer model.
There was an increase in the abundance of eccDNA with the progression of colorectal cancer. The genes associated with these eccDNA molecules were primarily related to signaling pathways involved in tumor development and metastasis. Our analysis also revealed that eccDNA abundance positively correlates with gene expression, and eccDNA derived from specific genes has potential value for the early diagnosis of tumors.
This study revealed a connection between eccDNA and colorectal cancer progression and highlights the clinical potential of eccDNA for the early diagnosis of colorectal cancer.
染色体外环状DNA（eccDNA）在肿瘤诊断中具有潜力，特别是在提高诊断准确性和早期癌症检测方面；然而，其在临床实践中的应用仍存在许多挑战。
我们对结直肠癌进展不同阶段的临床样本进行了Circle-Seq分析，以研究结直肠癌进展过程中eccDNA的动态变化。我们使用断点特异性PCR来验证通过Circle-Seq鉴定的候选eccDNA。结果在AOM/DSS诱导的结直肠癌模型中得到进一步验证。
随着结直肠癌的进展，eccDNA的丰度增加。与这些eccDNA分子相关的基因主要与肿瘤发生和转移所涉及的信号通路有关。我们的分析还表明，eccDNA丰度与基因表达呈正相关，并且源自特定基因的eccDNA对肿瘤的早期诊断具有潜在价值。
本研究揭示了eccDNA与结直肠癌进展之间的联系，并突出了eccDNA在结直肠癌早期诊断中的临床潜力。
