Yu Panling, Pan Xueyu, Chen Mingjie, Ma Jianshuai, Xu Baoting, Zhao Yan
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
Food Chem X. 2024 Jul 14;23:101657. doi: 10.1016/j.fochx.2024.101657. eCollection 2024 Oct 30.
Dietary fiber (DF) is an important active polysaccharide in . Obesity can lead to a wide range of diseases. In this work, we investigated the in vitro lipid-lowering effect of soluble dietary fiber (SDF) from , aiming to provide a basis for the subsequent development of lipid-lowering products. Ultrasound-assisted enzymatic extraction (UAEE) of SDF from was performed. The optimal extraction parameters determined via single-factor experiments and response surface methodology (RSM) were as follows: Lywallzyme concentration, 1.0%; complex protease concentration, 1.2%; ultrasonication time, 35 min; and ultrasonication power, 150 W. In vitro lipid-lowering experiments revealed that the adsorption amount of cholesterol micelles by SDF was 11.91 mg/g. The binding amount and binding rate of sodium taurocholate were 3.73 mg/g and 42.47%, respectively, and those of sodium glycocholate were 3.43 mg/g and 39.12%, respectively. The pancreatic lipase inhibition rate reached 52.11%, and the type of inhibition was competitive. Therefore, SDF has good in vitro lipid-lowering ability.
膳食纤维（DF）是一种重要的活性多糖。肥胖会引发多种疾病。在本研究中，我们探究了[具体来源]中可溶性膳食纤维（SDF）的体外降脂作用，旨在为后续降脂产品的开发提供依据。采用超声辅助酶解法从[具体来源]中提取SDF。通过单因素实验和响应面法（RSM）确定的最佳提取参数如下：裂壁酶浓度1.0%；复合蛋白酶浓度1.2%；超声处理时间35分钟；超声功率150瓦。体外降脂实验表明，[具体来源]SDF对胆固醇微团的吸附量为11.91毫克/克。牛磺胆酸钠的结合量和结合率分别为3.73毫克/克和42.47%，甘氨胆酸钠的结合量和结合率分别为3.43毫克/克和39.12%。胰脂肪酶抑制率达到52.11%，抑制类型为竞争性抑制。因此，[具体来源]SDF具有良好的体外降脂能力。
